Linked Life Data

11679252

Source:http://linkedlifedata.com/resource/pubmed/id/11679252

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9-12
pubmed:dateCreated
2001-10-26
pubmed:abstractText
We developed a system for amperometric detection of Escherichia coli (E. coli) based on the integration of microelectromechanical systems (MEMS), self-assembled monolayers (SAMS), DNA hybridization, and enzyme amplification. Using MEMS technology, a detector array was fabricated which has multiple electrodes deposited on a Si wafer and was fully reusable. Using SAMs, a monolayer of the protein streptavidin was immobilized on the working electrode (Au) surface to capture rRNA from E. coli. Three different approaches can be used to immobilize streptavidin onto Au, direct adsorption of the protein on bare Au, binding the protein to a biotinylated thiol SAM on Au, and binding the protein to a biotinylated disulfide monolayer on Au. The biotinylated thiol approach yielded the best results. High specificity for E. coli was achieved using ssDNA-rRNA hybridization and high sensitivity was achieved using enzymatic amplification with peroxidase as the enzyme. The analysis protocol can be conducted with solution volumes on the order of a few microliters and completed in 40 min. The detection system was capable of detecting 1000 E. coli cells without polymerase chain reaction with high specificity for E. coli vs. the bacteria Bordetella bronchiseptica.
pubmed:keyword
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0956-5663
pubmed:author
pubmed:issnType
Print
pubmed:volume
16
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
745-55
pubmed:dateRevised
2009-7-14
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
A MEMS based amperometric detector for E. coli bacteria using self-assembled monolayers.
pubmed:affiliation
Department of Biomedical Engineering, University of California at Los Angeles, 7523 Boelter Hall, 405 Hilgard Avenue, Los Angeles, CA 90095-1595, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.