Linked Life Data

11678634

Source:http://linkedlifedata.com/resource/pubmed/id/11678634

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2001-10-26
pubmed:abstractText
The molecular properties of fibrin-based matrices, such as fibrillar structure and covalent modifications with adhesion domains, influence the angiogenic behavior of human umbilical vein endothelial cells (HUVECs) in vitro. The fibrillar structure of fibrin-based matrices was influenced by pH but not by covalent incorporation of exogenous adhesion domains. Native fibrin-based matrices polymerized at pH 10 formed organized and longitudinally oriented fibrin fibrils, which provided a good angiogenic substrate for endothelial cells. Furthermore, upon covalent incorporation of the model ligand L1Ig6, which binds to the integrin most prominently expressed on the surface of angiogenic endothelial cells, alpha(v)beta3, these matrices became angiogenesis-promoting when polymerized at physiological pH. The amount of incorporation of L1Ig6 into the matrices depended on the fibrinogen concentration on all three fibrin chains. Soluble forms of L1Ig6 diffused rapidly out of the matrix. Most important, L1Ig6-modified matrices were very specific in inducing the angiogenic phenotype of HUVECs, whereas control cells did not differentiate on these matrices. Our results indicate that artificial extracellular matrices can influence cell behavior in two ways. One way is based on the three-dimensional fibril structure of the matrix molecules themselves, and the other is due to providing specific binding sites for direct cell-matrix interactions that lead to the activation of second-messenger cascades and thus promoting angiogenic differentiation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0026-2862
pubmed:author
pubmed:copyrightInfo
Copyright 2001 Academic Press.
pubmed:issnType
Print
pubmed:volume
62
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
315-26
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed-meshheading:11678634-Binding Sites, pubmed-meshheading:11678634-Cell Adhesion, pubmed-meshheading:11678634-Cell Division, pubmed-meshheading:11678634-Cells, Cultured, pubmed-meshheading:11678634-Cloning, Molecular, pubmed-meshheading:11678634-Endothelium, Vascular, pubmed-meshheading:11678634-Escherichia coli, pubmed-meshheading:11678634-Extracellular Matrix, pubmed-meshheading:11678634-Fibrin, pubmed-meshheading:11678634-Gels, pubmed-meshheading:11678634-Gene Expression, pubmed-meshheading:11678634-Humans, pubmed-meshheading:11678634-Hydrogen-Ion Concentration, pubmed-meshheading:11678634-Integrins, pubmed-meshheading:11678634-Leukocyte L1 Antigen Complex, pubmed-meshheading:11678634-Ligands, pubmed-meshheading:11678634-Membrane Glycoproteins, pubmed-meshheading:11678634-Microscopy, Fluorescence, pubmed-meshheading:11678634-Neovascularization, Physiologic, pubmed-meshheading:11678634-Neural Cell Adhesion Molecules, pubmed-meshheading:11678634-Phenotype, pubmed-meshheading:11678634-Protein Structure, Tertiary, pubmed-meshheading:11678634-Recombinant Proteins, pubmed-meshheading:11678634-Umbilical Veins
pubmed:year
2001
pubmed:articleTitle
Molecular properties of fibrin-based matrices for promotion of angiogenesis in vitro.
pubmed:affiliation
Institute for Biomedical Engineering and Department of Materials, ETH and, Zurich, Switzerland. hall@biomed.mat.ethz.ch
pubmed:publicationType
Journal Article