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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
2001-10-24
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pubmed:databankReference |
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pubmed:abstractText |
The 23-kDa proteolipid subunit of mouse vacuolar-type proton-translocating ATPase (V-ATPase) was predicted to be a hydrophobic polypeptide of 205 amino acid residues with five putative transmembrane segments. It exhibits sequence similarity to Vma16p of Saccharomyces cerevisiae and vha-4 of Caenorhabdittis elegans (83 and 84%, respectively). Southern blot analysis indicated that the proteolipid is encoded by a single gene, Atp6f, in the mouse genome. Atp6f was mapped to approximately 55 cM on chromosome 4, and its genomic organization is similar to that of the human gene: 8 exons separated by 7 introns, with boundaries matching the GT-AG rule. RNA blotting demonstrated that Atp6f is transcribed as 1.0- and 1.8-kb mRNAs in multiple tissues to varying degrees. The major transcription initiation sites are at -13 and -58 bp upstream of the translation initiation codon. The epitope-tagged 23-kDa protoelipid was localized in endomembrane organelles in CHO cells, as expected for a component of a vacuolar-type proton pump.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ATP6V0B protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Atp6v0b protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Subunits,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Vacuolar Proton-Translocating...
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0378-1119
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
22
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pubmed:volume |
274
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
93-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11675001-Alternative Splicing,
pubmed-meshheading:11675001-Amino Acid Sequence,
pubmed-meshheading:11675001-Animals,
pubmed-meshheading:11675001-Base Sequence,
pubmed-meshheading:11675001-CHO Cells,
pubmed-meshheading:11675001-Chromosome Mapping,
pubmed-meshheading:11675001-Cloning, Molecular,
pubmed-meshheading:11675001-Cricetinae,
pubmed-meshheading:11675001-DNA,
pubmed-meshheading:11675001-DNA, Complementary,
pubmed-meshheading:11675001-Exons,
pubmed-meshheading:11675001-Gene Expression,
pubmed-meshheading:11675001-Genes,
pubmed-meshheading:11675001-Introns,
pubmed-meshheading:11675001-Male,
pubmed-meshheading:11675001-Mice,
pubmed-meshheading:11675001-Mice, Inbred C57BL,
pubmed-meshheading:11675001-Mice, Inbred Strains,
pubmed-meshheading:11675001-Molecular Sequence Data,
pubmed-meshheading:11675001-Protein Subunits,
pubmed-meshheading:11675001-RNA, Messenger,
pubmed-meshheading:11675001-Sequence Alignment,
pubmed-meshheading:11675001-Sequence Analysis, DNA,
pubmed-meshheading:11675001-Sequence Homology, Amino Acid,
pubmed-meshheading:11675001-Sequence Homology, Nucleic Acid,
pubmed-meshheading:11675001-Tissue Distribution,
pubmed-meshheading:11675001-Transcription Initiation Site,
pubmed-meshheading:11675001-Vacuolar Proton-Translocating ATPases
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pubmed:year |
2001
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pubmed:articleTitle |
Mouse Atp6f, the gene encoding the 23-kDa proteolipid of vacuolar proton translocating ATPase.
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pubmed:affiliation |
Division of Biological Sciences, Institute of Scientific and Industrial Research, Osaka University, Mihogaoka 8-1, Ibaraki, Osaka 567-0047, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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