Linked Life Data

11673458

Source:http://linkedlifedata.com/resource/pubmed/id/11673458

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
51
pubmed:dateCreated
2001-12-17
pubmed:abstractText
Hepatic up-regulation of sterol carrier protein 2 (Scp2) in mice promotes hypersecretion of cholesterol into bile and gallstone formation in response to a lithogenic diet. We hypothesized that Scp2 deficiency may alter biliary lipid secretion and hepatic cholesterol metabolism. Male gallstone-susceptible C57BL/6 and C57BL/6(Scp2(-/-)) knockout mice were fed a standard chow or lithogenic diet. Hepatic biles were collected to determine biliary lipid secretion rates, bile flow, and bile salt pool size. Plasma lipoprotein distribution was investigated, and gene expression of cytosolic lipid-binding proteins, lipoprotein receptors, hepatic regulatory enzymes, and intestinal cholesterol absorption was measured. Compared with chow-fed wild-type animals, C57BL/6(Scp2(-/-)) mice had higher bile flow and lower bile salt secretion rates, decreased hepatic apolipoprotein expression, increased hepatic cholesterol synthesis, and up-regulation of liver fatty acid-binding protein. In addition, the bile salt pool size was reduced and intestinal cholesterol absorption was unaltered in C57BL/6(Scp2(-/-)) mice. When C57BL/6(Scp2(-/-)) mice were challenged with a lithogenic diet, a smaller increase of hepatic free cholesterol failed to suppress cholesterol synthesis and biliary cholesterol secretion increased to a much smaller extent than phospholipid and bile salt secretion. Scp2 deficiency did not prevent gallstone formation and may be compensated in part by hepatic up-regulation of liver fatty acid-binding protein. These results support a role of Scp2 in hepatic cholesterol metabolism, biliary lipid secretion, and intracellular cholesterol distribution.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
21
pubmed:volume
276
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
48058-65
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11673458-Animals, pubmed-meshheading:11673458-Bile, pubmed-meshheading:11673458-Body Weight, pubmed-meshheading:11673458-Carrier Proteins, pubmed-meshheading:11673458-Cholelithiasis, pubmed-meshheading:11673458-Cholesterol, pubmed-meshheading:11673458-Fatty Acid-Binding Proteins, pubmed-meshheading:11673458-Intestinal Absorption, pubmed-meshheading:11673458-Lipid Metabolism, pubmed-meshheading:11673458-Liver, pubmed-meshheading:11673458-Male, pubmed-meshheading:11673458-Mice, pubmed-meshheading:11673458-Mice, Inbred C57BL, pubmed-meshheading:11673458-Models, Animal, pubmed-meshheading:11673458-Neoplasm Proteins, pubmed-meshheading:11673458-Nerve Tissue Proteins, pubmed-meshheading:11673458-Organ Size, pubmed-meshheading:11673458-Plant Proteins, pubmed-meshheading:11673458-Reverse Transcriptase Polymerase Chain Reaction
pubmed:year
2001
pubmed:articleTitle
Disruption of the sterol carrier protein 2 gene in mice impairs biliary lipid and hepatic cholesterol metabolism.
pubmed:affiliation
Division of Gastroenterology, Department of Medicine I, Medical University of Lübeck, D-23538 Lübeck, Germany. michael.fuchs@medizin.uni-ulm.de
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't