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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2001-10-23
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pubmed:abstractText |
PU.1(+/-)Spi-B(-/-) mice exhibit reduced numbers of immature and mature B lymphocytes, which exhibit severe defects in response to BCR-mediated stimulation and poor survival. We found that expression of c-rel, a member of the Rel/NF-kappa B family, is dramatically reduced in PU.1(+/-)Spi-B(-/-) splenic B cells. Analysis of the murine c-rel promoter identified three PU.1/Spi-B binding sites critical for c-rel promoter activity. Furthermore, reintroduction of Rel protein restored wild-type B cell numbers to mice reconstituted with PU.1(+/-)Spi-B(-/-) bone marrow. These findings are the first to demonstrate that a member of the Rel/NF-kappa B family is directly regulated by Ets proteins and dissect the molecular basis for the function of two Ets factors, PU.1 and Spi-B, in promoting B lymphocyte survival.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-bcl-2,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-rel,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/SPIB protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/proto-oncogene protein Spi-1
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1074-7613
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
15
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
545-55
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:11672537-Animals,
pubmed-meshheading:11672537-B-Lymphocytes,
pubmed-meshheading:11672537-Binding Sites,
pubmed-meshheading:11672537-Bone Marrow Cells,
pubmed-meshheading:11672537-Bone Marrow Transplantation,
pubmed-meshheading:11672537-Cell Line,
pubmed-meshheading:11672537-Cell Survival,
pubmed-meshheading:11672537-Cells, Cultured,
pubmed-meshheading:11672537-DNA-Binding Proteins,
pubmed-meshheading:11672537-Down-Regulation,
pubmed-meshheading:11672537-Gene Expression Regulation,
pubmed-meshheading:11672537-Mice,
pubmed-meshheading:11672537-Mice, Knockout,
pubmed-meshheading:11672537-Promoter Regions, Genetic,
pubmed-meshheading:11672537-Proto-Oncogene Proteins,
pubmed-meshheading:11672537-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:11672537-Proto-Oncogene Proteins c-rel,
pubmed-meshheading:11672537-RNA, Messenger,
pubmed-meshheading:11672537-Spleen,
pubmed-meshheading:11672537-Trans-Activators,
pubmed-meshheading:11672537-Transcription Factors,
pubmed-meshheading:11672537-Transfection
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pubmed:year |
2001
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pubmed:articleTitle |
PU.1/Spi-B regulation of c-rel is essential for mature B cell survival.
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pubmed:affiliation |
Abramson Family Cancer Research Institute, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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