Source:http://linkedlifedata.com/resource/pubmed/id/11641609
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| rdf:type | |
| lifeskim:mentions |
umls-concept:C0004561,
umls-concept:C0015272,
umls-concept:C0017262,
umls-concept:C0023158,
umls-concept:C0040421,
umls-concept:C0134835,
umls-concept:C0221912,
umls-concept:C0750502,
umls-concept:C0870432,
umls-concept:C1171362,
umls-concept:C1264633,
umls-concept:C1419941,
umls-concept:C1515670,
umls-concept:C1555465,
umls-concept:C1704419,
umls-concept:C1705417,
umls-concept:C1749457
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| pubmed:issue |
1
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| pubmed:dateCreated |
2001-10-19
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| pubmed:abstractText |
Skin-homing T cells are defined by the expression of the cutaneous lymphocyte-associated antigen (CLA) which enables the cells to selectively bind to vascular endothelial E-selectin close to sites of cutaneous inflammation, an initial step in the effective extravasation from blood into the inflamed tissue. Essentially all CLA on T cells decorates the backbone of the P-selectin glycoprotein ligand-1 (PSGL-1). In this study we show that human peripheral blood B cells (PBBC) and tonsillar B cells (TBC) do not display PSGL-1 in fluorescence-activated cell sorter analysis using different murine monoclonal antibodies and polyclonal rabbit anti-PSGL-1 antiserum. A significant population of TBC, however, expresses a HECA-452-reactive epitope. These cells represent nonactivated IgM(+)/IgG(-) mature B lymphocytes. Up to 50% of the TBC in a given preparation strongly bind to E- and up to 79% to P-selectin. The shear stress resistance in a parallel-plate flow chamber system was high. Neuraminidase treatment of TBC totally and O-sialoglycoprotein endopeptidase partially diminished HECA-452 reactivity and reduced E- but not P-selectin ligand activities. Mocarhagin had no effect in the assays. The data suggest a different ligand for P-selectin and a distinct glycoprotein carrier for the E-selectin ligand as compared to T cells or other leukocytes. Adhesion to P-selectin, however, still required sulfation of the ligand for function. Western blots of TBC cell lysates detected a >240-kD HECA-452-reactive material that was resistant to reducing conditions. Anti-PSGL-1 did not reveal immunoreactive material in these cell lysates. B cell activation did neither significantly change HECA positivity nor induce PSGL-1 expression. Cultured, activated TBC, however, maintained expression of the integrin alpha4beta7. Human peripheral blood B cells had similar cell surface characteristics to TBC. Our observations suggest that several adhesion molecules may be involved in B cell homing which include CLA, the P-selectin ligand, and structures such as alpha4beta7.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/CTAGE1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin M,
http://linkedlifedata.com/resource/pubmed/chemical/Ligands,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Metalloendopeptidases,
http://linkedlifedata.com/resource/pubmed/chemical/Neuraminidase,
http://linkedlifedata.com/resource/pubmed/chemical/O-sialoglycoprotein endopeptidase,
http://linkedlifedata.com/resource/pubmed/chemical/P-Selectin,
http://linkedlifedata.com/resource/pubmed/chemical/P-selectin ligand protein,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, B-Cell,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Lymphocyte Homing
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| pubmed:status |
MEDLINE
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| pubmed:month |
Sep
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| pubmed:issn |
1018-2438
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2001 S. Karger AG, Basel
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| pubmed:issnType |
Print
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| pubmed:volume |
126
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
78-90
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| pubmed:dateRevised |
2010-11-18
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| pubmed:meshHeading |
pubmed-meshheading:11641609-Antigens, Differentiation, T-Lymphocyte,
pubmed-meshheading:11641609-Antigens, Neoplasm,
pubmed-meshheading:11641609-B-Lymphocyte Subsets,
pubmed-meshheading:11641609-Blotting, Western,
pubmed-meshheading:11641609-Cell Adhesion,
pubmed-meshheading:11641609-Cell Differentiation,
pubmed-meshheading:11641609-Cell Movement,
pubmed-meshheading:11641609-Cells, Cultured,
pubmed-meshheading:11641609-Humans,
pubmed-meshheading:11641609-Immunoglobulin M,
pubmed-meshheading:11641609-Interphase,
pubmed-meshheading:11641609-Ligands,
pubmed-meshheading:11641609-Membrane Glycoproteins,
pubmed-meshheading:11641609-Metalloendopeptidases,
pubmed-meshheading:11641609-Neuraminidase,
pubmed-meshheading:11641609-P-Selectin,
pubmed-meshheading:11641609-Palatine Tonsil,
pubmed-meshheading:11641609-Receptors, Antigen, B-Cell,
pubmed-meshheading:11641609-Receptors, Lymphocyte Homing
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| pubmed:year |
2001
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| pubmed:articleTitle |
Tonsillar B cells do not express PSGL-1, but a significant fraction displays the cutaneous lymphocyte antigen and exhibits effective E- and P-selectin ligand activity.
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| pubmed:affiliation |
Division of Dermatology, Harvard Skin Disease Research Center, Brigham and Women's Hospital, Boston, Mass, USA. dieter-armerding@aon.at
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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