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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2001-10-19
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pubmed:abstractText |
We screened for mutations in the gene of insulin-sensitive phosphodiesterase 3B (PDE3B), which regulates antilipolytic actions of insulin via reduction of intracellular cyclic AMP levels, in Japanese patients with type 2 diabetes mellitus and lipoatrophic diabetes mellitus using single-stranded conformation polymorphism analysis and Southern analysis and investigated frequencies of variable number of tandem repeats. A silent polymorphism at the Arg463 codon (AGG-->AGA) in exon 4 was identified after examining all 16 exons and exon-intron splicing junctions of the gene. This polymorphism was found in 53 of 100 subjects with type 2 diabetes mellitus, 2 of 5 lipoatrophic diabetic patients and 24 of 50 control subjects, without any significant difference in allele frequency between groups. An EcoRI restriction fragment length polymorphism was identified in patients with type 2 diabetes mellitus and control subjects, again with no differences in occurrence. The allelic distribution of two polymorphic tandem repeats sequences in introns 5 and 12 of the gene did not differ significantly between patients with type 2 diabetes mellitus and control subjects. In conclusion, alterations in the PDE3B gene are unlikely to contribute importantly to the pathogenesis of type 2 diabetes mellitus or lipoatrophic diabetes mellitus in Japan.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0168-8227
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
54
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
79-88
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:11640991-3',5'-Cyclic-AMP Phosphodiesterases,
pubmed-meshheading:11640991-Alleles,
pubmed-meshheading:11640991-Asian Continental Ancestry Group,
pubmed-meshheading:11640991-Blotting, Southern,
pubmed-meshheading:11640991-Body Mass Index,
pubmed-meshheading:11640991-Cholesterol,
pubmed-meshheading:11640991-Cyclic Nucleotide Phosphodiesterases, Type 3,
pubmed-meshheading:11640991-DNA Primers,
pubmed-meshheading:11640991-Diabetes Mellitus, Lipoatrophic,
pubmed-meshheading:11640991-Diabetes Mellitus, Type 2,
pubmed-meshheading:11640991-Dinucleotide Repeats,
pubmed-meshheading:11640991-Gene Frequency,
pubmed-meshheading:11640991-Hemoglobin A, Glycosylated,
pubmed-meshheading:11640991-Humans,
pubmed-meshheading:11640991-Introns,
pubmed-meshheading:11640991-Japan,
pubmed-meshheading:11640991-Polymerase Chain Reaction,
pubmed-meshheading:11640991-Polymorphism, Genetic,
pubmed-meshheading:11640991-Polymorphism, Single-Stranded Conformational,
pubmed-meshheading:11640991-Reference Values,
pubmed-meshheading:11640991-Triglycerides
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pubmed:year |
2001
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pubmed:articleTitle |
Analysis of the insulin-sensitive phosphodiesterase 3B gene in type 2 diabetes.
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pubmed:affiliation |
Department of Clinical Cell Biology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, 260-8670, Chiba, Japan.
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pubmed:publicationType |
Journal Article,
Comparative Study
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