Source:http://linkedlifedata.com/resource/pubmed/id/11607252
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2001-10-18
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| pubmed:abstractText |
1-Naphthylphthalamic acid (NPA) is a specific inhibitor of polar auxin transport that blocks carrier-mediated auxin efflux from plant cells. To allow identification of the NPA receptor thought to be part of the auxin efflux carrier, we have synthesized a tritiated, photolabile NPA analogue, 5'-azido-[3,6-3H2]NPA ([3H2]N3NPA). This analogue was used to identify NPA-binding proteins in fractions highly enriched for plasma membrane vesicles isolated from maize coleoptiles (Zea mays L.). Competition studies showed that binding of [3H2]N3NPA to maize plasma membrane vesicles was blocked by nonradioactive NPA but not by benzoic acid. After incubation of plasma membrane vesicles with [3H2]N3NPA and exposure to UV light, we observed specific photoaffinity labeling of a protein with an apparent molecular mass of 23 kDa. Pretreatment of the plasma membrane vesicles with indole-3-acetic acid or with the auxin-transport inhibitors NPA and 2,3,5-triiodobenzoic acid strongly reduced specific labeling of this protein. This 23-kDa protein was also labeled by addition of 5-azido-[7-3H]indole-3-acetic acid to plasma membranes prior to exposure to UV light. The 23-kDa protein was solubilized from plasma membranes by 1% Triton X-100. The possibility that this 23-kDa polypeptide is part of the auxin efflux carrier system is discussed.
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| pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11607252-11537412,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11607252-11537490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11607252-11607251,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11607252-16594060,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11607252-16661579,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11607252-16661939,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11607252-16663744,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11607252-16665662,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11607252-17734864,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11607252-1827995,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11607252-2555179,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11607252-2712285,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11607252-6847617,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11607252-942051
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:status |
PubMed-not-MEDLINE
|
| pubmed:month |
Jan
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| pubmed:issn |
0027-8424
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
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| pubmed:volume |
89
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
480-4
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| pubmed:dateRevised |
2010-9-14
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| pubmed:year |
1992
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| pubmed:articleTitle |
5'-Azido-[3,6-3H2]-1-napthylphthalamic acid, a photoactivatable probe for naphthylphthalamic acid receptor proteins from higher plants: identification of a 23-kDa protein from maize coleoptile plasma membranes.
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| pubmed:affiliation |
Max-Planck-Institut fur Zuchtungsforschung, Koln, Federal Republic of Germany.
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| pubmed:publicationType |
Journal Article
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