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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
50
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pubmed:dateCreated |
2001-12-12
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pubmed:abstractText |
The phenotypic modulation of vascular smooth muscle cells (VSMC) plays a central role in the pathogenesis of arteriosclerosis. Aortic preferentially expressed gene-1 (APEG-1), a VSMC-specific gene, is expressed highly in differentiated but not in dedifferentiated VSMC. Previously, we identified an E-box element in the mouse APEG-1 proximal promoter, which is essential for VSMC reporter activity. In this study, we investigated the role of upstream stimulatory factors (USF) in the regulation of APEG-1 transcription via this E-box element. By electrophoretic mobility shift assays, recombinant USF1 and USF2 homo- and heterodimers bound specifically to the APEG-1 E-box. Nuclear extracts prepared from primary cultures of rat aortic smooth muscle cells exhibited specific USF1 and USF2 binding to the APEG-1 E-box. To investigate the binding properties of USF during VSMC differentiation, nuclear extracts were prepared from the neural crest cell line, MONC-1, which differentiates into VSMC in culture. Maximal USF1 and USF2 protein levels and binding to the APEG-1 E-box occurred 3 h after the differentiation of MONC-1 cells was initiated. Co-transfection experiments demonstrated that dominant negative USF repressed APEG-1 promoter activity, and USF1, but not USF2, transactivated the APEG-1 promoter. Our studies demonstrate that USF factors contribute to the regulation of APEG-1 expression and may influence the differentiation of VSMC.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
14
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pubmed:volume |
276
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
47658-63
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:11606591-Animals,
pubmed-meshheading:11606591-Aorta,
pubmed-meshheading:11606591-Aorta, Thoracic,
pubmed-meshheading:11606591-Blotting, Northern,
pubmed-meshheading:11606591-Blotting, Western,
pubmed-meshheading:11606591-Cell Differentiation,
pubmed-meshheading:11606591-Cell Nucleus,
pubmed-meshheading:11606591-Cells, Cultured,
pubmed-meshheading:11606591-DNA-Binding Proteins,
pubmed-meshheading:11606591-Dimerization,
pubmed-meshheading:11606591-Dose-Response Relationship, Drug,
pubmed-meshheading:11606591-Gene Expression Regulation,
pubmed-meshheading:11606591-Genes, Dominant,
pubmed-meshheading:11606591-Luciferases,
pubmed-meshheading:11606591-Male,
pubmed-meshheading:11606591-Muscle, Smooth,
pubmed-meshheading:11606591-Muscle, Smooth, Vascular,
pubmed-meshheading:11606591-Phenotype,
pubmed-meshheading:11606591-Promoter Regions, Genetic,
pubmed-meshheading:11606591-Protein Binding,
pubmed-meshheading:11606591-Protein Biosynthesis,
pubmed-meshheading:11606591-RNA, Messenger,
pubmed-meshheading:11606591-Rats,
pubmed-meshheading:11606591-Rats, Sprague-Dawley,
pubmed-meshheading:11606591-Time Factors,
pubmed-meshheading:11606591-Transcription, Genetic,
pubmed-meshheading:11606591-Transcription Factors,
pubmed-meshheading:11606591-Transfection,
pubmed-meshheading:11606591-Upstream Stimulatory Factors
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pubmed:year |
2001
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pubmed:articleTitle |
Upstream stimulatory factors regulate aortic preferentially expressed gene-1 expression in vascular smooth muscle cells.
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pubmed:affiliation |
Pulmonary and Critical Care and Cardiovascular Divisions, Brigham and Women's Hospital, 75 Francis Street, Boston, MA 02115, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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