| pubmed-article:11602660 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11602660 | lifeskim:mentions | umls-concept:C0024554 | lld:lifeskim |
| pubmed-article:11602660 | lifeskim:mentions | umls-concept:C0034721 | lld:lifeskim |
| pubmed-article:11602660 | lifeskim:mentions | umls-concept:C0034693 | lld:lifeskim |
| pubmed-article:11602660 | lifeskim:mentions | umls-concept:C0027882 | lld:lifeskim |
| pubmed-article:11602660 | lifeskim:mentions | umls-concept:C0229048 | lld:lifeskim |
| pubmed-article:11602660 | lifeskim:mentions | umls-concept:C1514758 | lld:lifeskim |
| pubmed-article:11602660 | lifeskim:mentions | umls-concept:C0596235 | lld:lifeskim |
| pubmed-article:11602660 | lifeskim:mentions | umls-concept:C1709059 | lld:lifeskim |
| pubmed-article:11602660 | lifeskim:mentions | umls-concept:C0521116 | lld:lifeskim |
| pubmed-article:11602660 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:11602660 | pubmed:dateCreated | 2001-10-16 | lld:pubmed |
| pubmed-article:11602660 | pubmed:abstractText | Modulation of voltage-activated Ca2+ channels by adenosine was investigated in male rat major pelvic ganglion (MPG) neurons by using the whole-cell variant of the patch-clamp technique. Adenosine inhibited high voltage-activated (HVA) Ca2+ currents in a concentration-dependent manner with an EC50 of 313 nM and a maximal inhibition of 36%, respectively. Inhibition of HVA Ca2+ currents in adrenergic and cholinergic MPG neurons was similar. Adenosine did not modulate T-type Ca2+ channels present in adrenergic MPG neurons. Reverse transcription-polymerase chain reaction analysis indicated that MPG neurons express mRNAs encoding A1 and A2a receptors. Ca2+ current inhibition by adenosine was mimicked by N6-cyclopentyladenosine, an A1-selective agonist (EC50 = 63 nM) and prevented by 100 nM 8-cyclopentyl-1,3-dipropylxanthine, an A1-selective antagonist. Conversely, CGS 21680, an A2a-selective agonist, displayed a relatively low potency (EC50 = 2200 nM) for inhibiting Ca2+ currents. The action of adenosine was significantly attenuated by 2 mM guanosine-5'-thiodiphosphate or 500 ng/ml pertussis toxin. The voltage dependence of adenosine-induced current inhibition was evident by 1) a bell-shaped profile between the current inhibition and test potentials, 2) kinetic slowing in the presence of agonist, and 3) relief of the current inhibition by a conditioning prepulse to +80 mV. Finally, 1 microM omega-conotoxin GVIA occluded adenosine-induced current inhibition. Taken together, we concluded that adenosine inhibits N-type Ca2+ currents by activation of A1 receptors via a voltage-dependent and PTX-sensitive pathway in rat MPG neurons. Our data may explain how adenosine acts as an inhibitory modulator of ganglionic and neuromuscular transmission in the pelvic plexus. | lld:pubmed |
| pubmed-article:11602660 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11602660 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11602660 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11602660 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11602660 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11602660 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11602660 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11602660 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11602660 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11602660 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11602660 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11602660 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11602660 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11602660 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11602660 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11602660 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11602660 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:11602660 | pubmed:issn | 0022-3565 | lld:pubmed |
| pubmed-article:11602660 | pubmed:author | pubmed-author:LeeJ WJW | lld:pubmed |
| pubmed-article:11602660 | pubmed:author | pubmed-author:ParkK SKS | lld:pubmed |
| pubmed-article:11602660 | pubmed:author | pubmed-author:LEVI VIV | lld:pubmed |
| pubmed-article:11602660 | pubmed:author | pubmed-author:LeeB SBS | lld:pubmed |
| pubmed-article:11602660 | pubmed:author | pubmed-author:IkedaS RSR | lld:pubmed |
| pubmed-article:11602660 | pubmed:author | pubmed-author:JeongS WSW | lld:pubmed |
| pubmed-article:11602660 | pubmed:author | pubmed-author:KongI DID | lld:pubmed |
| pubmed-article:11602660 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11602660 | pubmed:volume | 299 | lld:pubmed |
| pubmed-article:11602660 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11602660 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11602660 | pubmed:pagination | 501-8 | lld:pubmed |
| pubmed-article:11602660 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
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| pubmed-article:11602660 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11602660 | pubmed:articleTitle | Modulation of N-type Ca2+ currents by A1-adenosine receptor activation in male rat pelvic ganglion neurons. | lld:pubmed |
| pubmed-article:11602660 | pubmed:affiliation | Department of Physiology and Institute of Basic Medical Science, Yonsei University Wonju College of Medicine, Wonju, Kangwon-Do, Korea. | lld:pubmed |
| pubmed-article:11602660 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11602660 | lld:pubmed |
