Source:http://linkedlifedata.com/resource/pubmed/id/11602660
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2001-10-16
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| pubmed:abstractText |
Modulation of voltage-activated Ca2+ channels by adenosine was investigated in male rat major pelvic ganglion (MPG) neurons by using the whole-cell variant of the patch-clamp technique. Adenosine inhibited high voltage-activated (HVA) Ca2+ currents in a concentration-dependent manner with an EC50 of 313 nM and a maximal inhibition of 36%, respectively. Inhibition of HVA Ca2+ currents in adrenergic and cholinergic MPG neurons was similar. Adenosine did not modulate T-type Ca2+ channels present in adrenergic MPG neurons. Reverse transcription-polymerase chain reaction analysis indicated that MPG neurons express mRNAs encoding A1 and A2a receptors. Ca2+ current inhibition by adenosine was mimicked by N6-cyclopentyladenosine, an A1-selective agonist (EC50 = 63 nM) and prevented by 100 nM 8-cyclopentyl-1,3-dipropylxanthine, an A1-selective antagonist. Conversely, CGS 21680, an A2a-selective agonist, displayed a relatively low potency (EC50 = 2200 nM) for inhibiting Ca2+ currents. The action of adenosine was significantly attenuated by 2 mM guanosine-5'-thiodiphosphate or 500 ng/ml pertussis toxin. The voltage dependence of adenosine-induced current inhibition was evident by 1) a bell-shaped profile between the current inhibition and test potentials, 2) kinetic slowing in the presence of agonist, and 3) relief of the current inhibition by a conditioning prepulse to +80 mV. Finally, 1 microM omega-conotoxin GVIA occluded adenosine-induced current inhibition. Taken together, we concluded that adenosine inhibits N-type Ca2+ currents by activation of A1 receptors via a voltage-dependent and PTX-sensitive pathway in rat MPG neurons. Our data may explain how adenosine acts as an inhibitory modulator of ganglionic and neuromuscular transmission in the pelvic plexus.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channel Blockers,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channels, N-Type,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Guanosine Diphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Pertussis Toxin,
http://linkedlifedata.com/resource/pubmed/chemical/Purinergic P1 Receptor Agonists,
http://linkedlifedata.com/resource/pubmed/chemical/Thionucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Virulence Factors, Bordetella,
http://linkedlifedata.com/resource/pubmed/chemical/guanosine 5'-O-(2-thiodiphosphate),
http://linkedlifedata.com/resource/pubmed/chemical/omega-Conotoxins
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| pubmed:status |
MEDLINE
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| pubmed:month |
Nov
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| pubmed:issn |
0022-3565
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
299
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
501-8
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| pubmed:dateRevised |
2010-11-18
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| pubmed:meshHeading |
pubmed-meshheading:11602660-Adrenergic Fibers,
pubmed-meshheading:11602660-Animals,
pubmed-meshheading:11602660-Calcium,
pubmed-meshheading:11602660-Calcium Channel Blockers,
pubmed-meshheading:11602660-Calcium Channels, N-Type,
pubmed-meshheading:11602660-Cholinergic Fibers,
pubmed-meshheading:11602660-DNA Primers,
pubmed-meshheading:11602660-Electrophysiology,
pubmed-meshheading:11602660-GTP-Binding Proteins,
pubmed-meshheading:11602660-Ganglia, Spinal,
pubmed-meshheading:11602660-Guanosine Diphosphate,
pubmed-meshheading:11602660-Male,
pubmed-meshheading:11602660-Membrane Potentials,
pubmed-meshheading:11602660-Neurons,
pubmed-meshheading:11602660-Pertussis Toxin,
pubmed-meshheading:11602660-Purinergic P1 Receptor Agonists,
pubmed-meshheading:11602660-Rats,
pubmed-meshheading:11602660-Rats, Sprague-Dawley,
pubmed-meshheading:11602660-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:11602660-Thionucleotides,
pubmed-meshheading:11602660-Virulence Factors, Bordetella,
pubmed-meshheading:11602660-omega-Conotoxins
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| pubmed:year |
2001
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| pubmed:articleTitle |
Modulation of N-type Ca2+ currents by A1-adenosine receptor activation in male rat pelvic ganglion neurons.
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| pubmed:affiliation |
Department of Physiology and Institute of Basic Medical Science, Yonsei University Wonju College of Medicine, Wonju, Kangwon-Do, Korea.
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| pubmed:publicationType |
Journal Article
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