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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
Pt 2
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pubmed:dateCreated |
2001-10-15
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pubmed:abstractText |
1. Cellular responses to GABA(A) receptor activation were studied in developing cerebellar Purkinje neurones (PNs) in brain slices obtained from 2- to 22-day-old rats. Two-photon fluorescence imaging of fura-2-loaded cells and perforated-patch recordings were used to monitor intracellular Ca2+ transients and to estimate the reversal potential of GABA-induced currents, respectively. 2. During the 1st postnatal week, focal application of GABA or the GABA(A) receptor agonist muscimol evoked transient increases in [Ca2+]i in immature PNs. These Ca2+ transients were reversibly abolished by the GABA(A) receptor antagonist bicuculline and by Ni2+, a blocker of voltage-activated Ca2+ channels. 3. Perforated-patch recordings were used to measure the reversal potential of GABA-evoked currents (E(GABA)) at different stages of development. It was found that E(GABA) was about -44 mV at postnatal day 3 (P3), it shifted to gradually more negative values during the 1st week and finally equilibrated at -87 mV at around the end of the 2nd postnatal week. This transition was well described by a sigmoidal function. The largest change in E(GABA) was -7 mV x day(-1), which occurred at around P6. 4. The transition in GABA-mediated signalling occurs during a period in which striking changes in PN morphology and synaptic connectivity are known to take place. Since such changes were shown to be Ca2+ dependent, we propose that GABA-evoked Ca2+ signalling is one of the critical determinants for the normal development of cerebellar PNs.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11600678-10191302,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11600678-10359832,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11600678-10380077,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11600678-10514814,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11600678-11050110,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11600678-1313949,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11600678-1403091,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11600678-1672071,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11600678-1709263,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/11600678-1822551,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/11600678-9930699
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0022-3751
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
536
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
429-37
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:11600678-Animals,
pubmed-meshheading:11600678-Anti-Bacterial Agents,
pubmed-meshheading:11600678-Bicuculline,
pubmed-meshheading:11600678-Calcium,
pubmed-meshheading:11600678-Calcium Signaling,
pubmed-meshheading:11600678-Cerebellum,
pubmed-meshheading:11600678-GABA Antagonists,
pubmed-meshheading:11600678-Gramicidin,
pubmed-meshheading:11600678-Membrane Potentials,
pubmed-meshheading:11600678-Nickel,
pubmed-meshheading:11600678-Patch-Clamp Techniques,
pubmed-meshheading:11600678-Purkinje Cells,
pubmed-meshheading:11600678-Rats,
pubmed-meshheading:11600678-Rats, Wistar,
pubmed-meshheading:11600678-Receptors, GABA-A,
pubmed-meshheading:11600678-gamma-Aminobutyric Acid
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pubmed:year |
2001
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pubmed:articleTitle |
GABA-mediated Ca2+ signalling in developing rat cerebellar Purkinje neurones.
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pubmed:affiliation |
Abteilung Neurophysiologie, Max-Planck-Institut für Hirnforschung, 60528 Frankfurt, Germany. eilers@mpih-frankfurt.mpg.de
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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