Linked Life Data

11599442

Source:http://linkedlifedata.com/resource/pubmed/id/11599442

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2001-10-15
pubmed:abstractText
A Western blotting procedure with excretory/secretory antigens from Toxocara canis larvae was developed for immunodiagnosis of visceral larva migrans in mice. In this study, eighty Swiss albino mice were allotted into two groups of 40 each as control and experimental groups, and T. canis ova containing infective larvae were given to mice in the latter group to form visceral larva migrans. Blood samples were taken from 5 infected and 5 control mice on days 25, 30, 35, 40, 45, 50, 55, and 60 after infection. After bleeding, the mice were necropsied. Slides were prepared from their brain tissues and examined for visceral larva migrans. Following this procedure, their guts were also examined for intestinal parasites. Protein bands of excretory/secretory antigens of 2nd stage larvae of Toxocara canis were determined by using SDS-PAGE. Sera from the mice were tested by Western blotting and results were compared to the protein bands obtained by SDS-PAGE to determine specific bands. Specific protein bands for visceral larva migrans were determined as 24, 28, and 48 kDa according to our test results.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0341-6593
pubmed:author
pubmed:issnType
Print
pubmed:volume
108
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
390-2
pubmed:dateRevised
2007-7-24
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
Application of western blotting procedure for the immunodiagnosis of visceral larva migrans in mice by using excretory/secretory antigens.
pubmed:affiliation
Ankara University, Faculty of Veterinary Medicine, Ankara, Turkey.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't