Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2001-10-12
pubmed:abstractText
Using the pUT-miniTn5 vector system developed by the laboratory of K.N. Timmis, the Vitreoscilla hemoglobin gene (vgb) was integrated into the chromosomes of Pseudomonas aeruginosa and Burkholderia cepacia; Vitreoscilla hemoglobin (VHb) was expressed at 8.8 and 0.8 nmol/g wet weight of cells in the respective engineered strains. The vgb-bearing P. aeruginosa outgrew wild-type P. aeruginosa and degraded benzoic acid faster than the latter strain at both normal and low aeration. In contrast, the vgb-bearing B. cepacia strain had a growth advantage over the wild-type strain at ca. 90 ppm, but not at ca. 120 ppm 2,4-dinitrotoluene (DNT); no difference in DNT degradation was seen between the two strains at either normal or low aeration. The results demonstrate the practicality of enhancing bioremediation with vgb stably integrated into the chromosome, but also suggest that a minimal level of VHb expression is required for its beneficial effects to be seen.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1367-5435
pubmed:author
pubmed:issnType
Print
pubmed:volume
27
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
27-33
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:11598807-Bacterial Proteins, pubmed-meshheading:11598807-Benzoic Acid, pubmed-meshheading:11598807-Biodegradation, Environmental, pubmed-meshheading:11598807-Blotting, Southern, pubmed-meshheading:11598807-Burkholderia, pubmed-meshheading:11598807-Chromosomes, Bacterial, pubmed-meshheading:11598807-Conjugation, Genetic, pubmed-meshheading:11598807-DNA Transposable Elements, pubmed-meshheading:11598807-Dinitrobenzenes, pubmed-meshheading:11598807-Genetic Engineering, pubmed-meshheading:11598807-Genetic Vectors, pubmed-meshheading:11598807-Hemoglobins, pubmed-meshheading:11598807-Plasmids, pubmed-meshheading:11598807-Polymerase Chain Reaction, pubmed-meshheading:11598807-Pseudomonas, pubmed-meshheading:11598807-Truncated Hemoglobins, pubmed-meshheading:11598807-Vitreoscilla
pubmed:year
2001
pubmed:articleTitle
Chromosomal integration of the Vitreoscilla hemoglobin gene in Burkholderia and Pseudomonas for the purpose of producing stable engineered strains with enhanced bioremediating ability.
pubmed:affiliation
Biology Division, Department of Biological, Chemical, and Physical Sciences, Illinois Institute of Technology, IIT Center, Chicago, IL 60616, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't