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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
22
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pubmed:dateCreated |
2001-10-24
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pubmed:abstractText |
Orphan receptors for whom cognate ligands have not yet been identified form a large subclass within the nuclear receptor superfamily. To address one aspect of how they might regulate transcription, we analyzed the mode of interaction between the Drosophila orphan receptor FTZ-F1 (NR5A3) and a segmentation gene product Fushi tarazu (FTZ). Strong interaction between these two factors was detected by use of the mammalian one- and two-hybrid interaction assays without addition of ligand. This interaction required the AF-2 core and putative ligand-binding domain of FTZ-F1 and the LXXLL motif of FTZ. The requirement of these elements was further confirmed by examination of their target gene expression in Drosophila embryos and observation of a cuticle phenotype in transgenic fly lines that express mutated factors. In Drosophila cultured cells, FTZ is required for FTZ-F1 activation of a FTZ-F1 reporter gene. These results reveal a resemblance in the mode of interaction between FTZ-F1 and FTZ and that of nuclear receptor-coactivator and indicate that direct interaction is required for regulation of gene expression by FTZ-F1. Thus, we propose that FTZ may represent a category of LXXLL motif-dependent coactivators for nuclear receptors.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11592991-10207113,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11592991-10219237,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11592991-10322133,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11592991-10529899,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11592991-10891484,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11592991-11060234,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11592991-1348571,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11592991-1648450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11592991-1709303,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11592991-2113881,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11592991-2476281,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11592991-2495181,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11592991-2893285,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11592991-3000605,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11592991-3123316,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11592991-4079981,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11592991-6330566,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11592991-6421639,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11592991-7501014,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11592991-7705655,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11592991-7758108,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11592991-7760929,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11592991-7809147,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11592991-8095092,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11592991-8392478,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11592991-8521507,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11592991-8521508,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11592991-8521509,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11592991-8521510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11592991-8521511,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11592991-8538765,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11592991-8668198,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11592991-8852847,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11592991-8887679,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11592991-8923456,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11592991-8978054,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11592991-8978696,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11592991-9020363,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11592991-9020364,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11592991-9192646,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11592991-9192892,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11592991-9192902,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11592991-9338790,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11592991-9624051,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11592991-9653119,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11592991-9744270,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11592991-9784489,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11592991-9808622,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11592991-9808623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11592991-9875847
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Drosophila Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Fushi Tarazu Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Homeodomain Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Insect Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cytoplasmic and Nuclear,
http://linkedlifedata.com/resource/pubmed/chemical/Steroidogenic Factor 1,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/ftz protein, Drosophila,
http://linkedlifedata.com/resource/pubmed/chemical/nuclear hormone receptor FTZ-F1...
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
23
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pubmed:volume |
98
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
12403-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:11592991-Animals,
pubmed-meshheading:11592991-Drosophila,
pubmed-meshheading:11592991-Cells, Cultured,
pubmed-meshheading:11592991-Amino Acid Sequence,
pubmed-meshheading:11592991-Binding Sites,
pubmed-meshheading:11592991-Insect Proteins,
pubmed-meshheading:11592991-Molecular Sequence Data,
pubmed-meshheading:11592991-DNA-Binding Proteins,
pubmed-meshheading:11592991-Amino Acid Motifs,
pubmed-meshheading:11592991-Drosophila Proteins,
pubmed-meshheading:11592991-Transcription Factors,
pubmed-meshheading:11592991-Receptors, Cytoplasmic and Nuclear
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