Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 4
pubmed:dateCreated
2001-10-10
pubmed:abstractText
DNA was extracted from specimens derived from the calcaneus of the Tyrolean Ice Man under sterile conditions in a laboratory, where no DNA extractions and PCR experiments had been performed before. Agarose gel electrophoresis and ethidium bromide staining did not reveal any evidence of genomic DNA in the preparation obtained, indicating a high degree of DNA degradation. Nevertheless, we performed PCR amplifications with this sample using primer pairs specific for HLA class II alleles. HLA-DRB and DQB1 alleles were amplified in a nested PCR approach. In one of the reactions, we observed a distinct amplification product, which we directly sequenced. By comparing the obtained nucleotide sequence with a database of HLA alleles we assigned the HLA-DRB1*1402 type to the amplified sample. None of the investigators involved possesses this allele, indicating that no contamination with modern DNA had occurred. The HLA-DRB1*1402 allele is extremely rare in Europe, but is common in Inuits and South American Indians and has previously only once been identified in the laboratory.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0003-4800
pubmed:author
pubmed:issnType
Print
pubmed:volume
65
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
363-9
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:11592925-Alleles, pubmed-meshheading:11592925-Base Sequence, pubmed-meshheading:11592925-Calcaneus, pubmed-meshheading:11592925-DNA, pubmed-meshheading:11592925-Gene Frequency, pubmed-meshheading:11592925-Genes, MHC Class II, pubmed-meshheading:11592925-HLA-DQ Antigens, pubmed-meshheading:11592925-HLA-DQ beta-Chains, pubmed-meshheading:11592925-HLA-DR Antigens, pubmed-meshheading:11592925-HLA-DRB1 Chains, pubmed-meshheading:11592925-History, Ancient, pubmed-meshheading:11592925-Humans, pubmed-meshheading:11592925-Indians, North American, pubmed-meshheading:11592925-Inuits, pubmed-meshheading:11592925-Italy, pubmed-meshheading:11592925-Male, pubmed-meshheading:11592925-Molecular Sequence Data, pubmed-meshheading:11592925-Mummies, pubmed-meshheading:11592925-Phylogeny, pubmed-meshheading:11592925-Polymerase Chain Reaction, pubmed-meshheading:11592925-Sensitivity and Specificity
pubmed:year
2001
pubmed:articleTitle
An HLA class-II allele frequent in Eskimos and Amerindians is found in the Tyrolean Ice Man.
pubmed:affiliation
Department of Blood Group Serology, University of Vienna Medical School, Austria. gottfried.fischer@akh-wien.ac.at
pubmed:publicationType
Journal Article, Historical Article