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rdf:type |
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lifeskim:mentions |
umls-concept:C0008109,
umls-concept:C0017262,
umls-concept:C0031330,
umls-concept:C0033684,
umls-concept:C0185117,
umls-concept:C0205148,
umls-concept:C0206529,
umls-concept:C0303920,
umls-concept:C0332307,
umls-concept:C0443199,
umls-concept:C1280500,
umls-concept:C1880022,
umls-concept:C2911684
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pubmed:issue |
3
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pubmed:dateCreated |
2001-10-9
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pubmed:abstractText |
Metabotropic glutamate receptor 5 (mGluR5) can modulate synaptic transmission by increasing intracellular Ca2+ and it plays a role in several forms of synaptic plasticity. We have constructed a fusion of human mGluR5 and green fluorescent protein (mGluR5-GFP). Expression of mGluR5-GFP in clonal cell lines yielded a functional fluorescent receptor with pharmacological profiles similar to wild-type mGluR5. mGluR5-GFP coimmunoprecipitated with Homer-1c, indicating that addition of GFP to the C-terminal did not prevent Homer binding. Coexpression of wild-type mGluR5 or mGluR5-GFP with Homer 1c, but not Homer-1a, resulted in reduced receptor surface localization and the formation of intracellular clusters. Neither Homer-1a nor Homer-1c had any effect on mGluR1 or mGluR1-GFP distribution. mGluR5-GFP expressed alone or in combination with Homer-1a formed dimers in HEK cells. Coexpression with Homer-1c, however, prevented mGluR5-GFP dimerization. Neither Homer altered the agonist profiles of mGluR5 or mGluR5-GFP. These data indicate that the functional expression of mGluR5 is regulated by Homer-1c and demonstrate that mGluR5-GFP provides a useful tool to study the molecular pharmacology and cell biology of mGluRs in real-time.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/6-methyl-2-(phenylethynyl)pyridine,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Excitatory Amino Acid Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Homer protein,
http://linkedlifedata.com/resource/pubmed/chemical/Luminescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Neuropeptides,
http://linkedlifedata.com/resource/pubmed/chemical/Pyridines,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Metabotropic Glutamate,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/metabotropic glutamate receptor 5
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1044-7431
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2001 Academic Press.
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pubmed:issnType |
Print
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pubmed:volume |
18
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
296-306
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11591130-Animals,
pubmed-meshheading:11591130-CHO Cells,
pubmed-meshheading:11591130-Carrier Proteins,
pubmed-meshheading:11591130-Cricetinae,
pubmed-meshheading:11591130-Dimerization,
pubmed-meshheading:11591130-Dose-Response Relationship, Drug,
pubmed-meshheading:11591130-Excitatory Amino Acid Antagonists,
pubmed-meshheading:11591130-Green Fluorescent Proteins,
pubmed-meshheading:11591130-Humans,
pubmed-meshheading:11591130-Immunoblotting,
pubmed-meshheading:11591130-Luminescent Proteins,
pubmed-meshheading:11591130-Neuropeptides,
pubmed-meshheading:11591130-Precipitin Tests,
pubmed-meshheading:11591130-Pyridines,
pubmed-meshheading:11591130-Receptors, Metabotropic Glutamate,
pubmed-meshheading:11591130-Recombinant Fusion Proteins,
pubmed-meshheading:11591130-Signal Transduction,
pubmed-meshheading:11591130-Transfection
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pubmed:year |
2001
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pubmed:articleTitle |
Characterization of a metabotropic glutamate receptor type 5-green fluorescent protein chimera (mGluR5-GFP): pharmacology, surface expression, and differential effects of Homer-1a and Homer-1c.
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pubmed:affiliation |
MRC Centre for Synaptic Plasticity, University Walk, Bristol, BS8 1TD, United Kingdom.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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