Source:http://linkedlifedata.com/resource/pubmed/id/11590227
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
10
|
| pubmed:dateCreated |
2001-10-8
|
| pubmed:abstractText |
The mono-(dimethylaminoethyl) succinyl (MDMAES) ester is a new derivative for rapid, mild, and sensitive electrospray ionization tandem mass spectrometry (ESI-MS/MS) analysis of cholesterol and dehydrocholesterol. It is an order of magnitude more sensitive than the previous most practical alternative, the N-methylpyridyl ether derivative. The MDMAES derivative was used to develop a rapid screening procedure for the biochemical diagnosis of Smith-Lemli-Opitz syndrome (SLOS) by measuring the dehydrocholesterol/cholesterol ratio in plasma (5 microl) and plasma spotted onto filter paper. Details of the synthesis of [25,26,26,26,27,27,27-(2)H7]-7-dehydrocholesterol, used as a standard for quantitation, are included. The measurement of total sterols as MDMAES esters, after base hydrolysis of plasma, afforded a dehydrocholesterol/cholesterol ratio of 0.05-2.95 for SLOS patient samples (n = 5) compared with 0.001-0.003 for normal adult controls (n = 20). Direct hexane extraction of plasma without base hydrolysis enabled the measurement of free sterols with a total sample analysis time of <1 h. The free dehydrocholesterol/cholesterol ratio was 0.10-4.47 for SLOS patient samples (n = 5) and 0.003-0.011 for normal adult controls (n = 20).
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
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| pubmed:issn |
0022-2275
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
42
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1699-705
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:11590227-Adult,
pubmed-meshheading:11590227-Cholesterol,
pubmed-meshheading:11590227-Dehydrocholesterols,
pubmed-meshheading:11590227-Gas Chromatography-Mass Spectrometry,
pubmed-meshheading:11590227-Humans,
pubmed-meshheading:11590227-Mass Screening,
pubmed-meshheading:11590227-Sensitivity and Specificity,
pubmed-meshheading:11590227-Smith-Lemli-Opitz Syndrome,
pubmed-meshheading:11590227-Spectrometry, Mass, Electrospray Ionization,
pubmed-meshheading:11590227-Time Factors
|
| pubmed:year |
2001
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| pubmed:articleTitle |
A rapid screening procedure for cholesterol and dehydrocholesterol by electrospray ionization tandem mass spectrometry.
|
| pubmed:affiliation |
Department of Chemical Pathology, Women's and Children's Hospital, 72 King William Road, North Adelaide, South Australia 5006, Australia. david.johnson@adelaide.edu.au
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| pubmed:publicationType |
Journal Article
|