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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
20
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pubmed:dateCreated |
2001-10-5
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pubmed:abstractText |
Heterotetrameric adaptor complexes vesiculate donor membranes. One of the adaptor protein complexes, AP-3, is present in two forms; one form is expressed in all tissues of the body, whereas the other is restricted to brain. Mice lacking both the ubiquitous and neuronal forms of AP-3 exhibit neurological disorders that are not observed in mice that are mutant only in the ubiquitous form. To begin to understand the role of neuronal AP-3 in neurological disease, we investigated its function in in vitro assays as well as its localization in neural tissue. In the presence of GTPgammaS both ubiquitous and neuronal forms of AP-3 can bind to purified synaptic vesicles. However, only the neuronal form of AP-3 can produce synaptic vesicles from endosomes in vitro. We also identified that the expression of neuronal AP-3 is limited to varicosities of neuronal-like processes and is expressed in most axons of the brain. Although the AP-2/clathrin pathway is the major route of vesicle production and the relatively minor neuronal AP-3 pathway is not necessary for viability, the absence of the latter could lead to the neurological abnormalities seen in mice lacking the expression of AP-3 in brain. In this study we have identified the first brain-specific function for a neuronal adaptor complex.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Vesicular...,
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Guanosine 5'-O-(3-Thiotriphosphate),
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Monomeric Clathrin Assembly Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Isoforms,
http://linkedlifedata.com/resource/pubmed/chemical/R-SNARE Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/clathrin assembly protein AP180
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1529-2401
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
15
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pubmed:volume |
21
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
8034-42
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:11588176-Adaptor Proteins, Vesicular Transport,
pubmed-meshheading:11588176-Adenosine Triphosphate,
pubmed-meshheading:11588176-Animals,
pubmed-meshheading:11588176-Antibodies,
pubmed-meshheading:11588176-Axons,
pubmed-meshheading:11588176-Brain,
pubmed-meshheading:11588176-Brain Chemistry,
pubmed-meshheading:11588176-Carrier Proteins,
pubmed-meshheading:11588176-Cell Line,
pubmed-meshheading:11588176-Cell-Free System,
pubmed-meshheading:11588176-Cytosol,
pubmed-meshheading:11588176-Endosomes,
pubmed-meshheading:11588176-Female,
pubmed-meshheading:11588176-Guanosine 5'-O-(3-Thiotriphosphate),
pubmed-meshheading:11588176-Humans,
pubmed-meshheading:11588176-Membrane Proteins,
pubmed-meshheading:11588176-Mice,
pubmed-meshheading:11588176-Mice, Mutant Strains,
pubmed-meshheading:11588176-Monomeric Clathrin Assembly Proteins,
pubmed-meshheading:11588176-Neurons,
pubmed-meshheading:11588176-Organ Specificity,
pubmed-meshheading:11588176-PC12 Cells,
pubmed-meshheading:11588176-Protein Isoforms,
pubmed-meshheading:11588176-R-SNARE Proteins,
pubmed-meshheading:11588176-Rats,
pubmed-meshheading:11588176-Rats, Sprague-Dawley,
pubmed-meshheading:11588176-Synaptic Vesicles
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pubmed:year |
2001
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pubmed:articleTitle |
The neuronal form of adaptor protein-3 is required for synaptic vesicle formation from endosomes.
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pubmed:affiliation |
Department of Biochemistry and Biophysics, University of California, San Francisco, California 94143-0448, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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