Linked Life Data

11587855

Source:http://linkedlifedata.com/resource/pubmed/id/11587855

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2001-10-5
pubmed:databankReference
pubmed:abstractText
Periodontal ligament (PDL) is one of the most important tissues in maintaining the homeostasis of tooth and tooth-supporting tissue, periodontium. In this study, we investigated the expression profile of active genes in the human PDL obtained by collecting sequences with a 3'-directed cDNA library, which faithfully represents the composition of the mRNA population. We succeeded in obtaining a total of 1752 cDNA sequences by sequencing randomly selected clones and identified a total of 1318 different species as gene signatures (GS) by their sequence identity, 344 of which were known genes in the GenBank, and 974 of which were new genes. The resulting expression profile showed that collagen type I and type III were the most abundant genes and that osteogenesis-related proteins, such as SPARC/osteonectin and osteoblast specific factor 2, were highly expressed. By comparing the expression profile of PDL with 44 profiles similarly obtained with unrelated human cell/tissue, nine novel genes, which are probably expressed specifically in PDL, were discovered. Among them, we cloned a full-length cDNA of GS5096, which is frequently expressed in freshly-isolated periodontal tissue. We found that it encodes a novel protein, which is a new member of the class I small leucine-rich repeat proteoglycan family, and designated it PLAP-1 (periodontal ligament associated protein-1). PLAP-1 mRNA expression was confirmed in in vitro-maintained PDL cells and was enhanced during the course of the cytodifferentiation of the PDL cells into mineralized tissue-forming cells such as osteoblasts and cementoblasts. These findings suggest the involvement of PLAP-1 in the mineralized matrix formation in PDL tissues.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0378-1119
pubmed:author
pubmed:issnType
Print
pubmed:day
19
pubmed:volume
275
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
279-86
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11587855-Amino Acid Sequence, pubmed-meshheading:11587855-Base Sequence, pubmed-meshheading:11587855-Carrier Proteins, pubmed-meshheading:11587855-Cell Division, pubmed-meshheading:11587855-Cells, Cultured, pubmed-meshheading:11587855-Cloning, Molecular, pubmed-meshheading:11587855-DNA, Complementary, pubmed-meshheading:11587855-Extracellular Matrix Proteins, pubmed-meshheading:11587855-Gene Expression Profiling, pubmed-meshheading:11587855-Gene Library, pubmed-meshheading:11587855-Humans, pubmed-meshheading:11587855-Molecular Sequence Data, pubmed-meshheading:11587855-Periodontal Ligament, pubmed-meshheading:11587855-RNA, Messenger, pubmed-meshheading:11587855-Sequence Alignment, pubmed-meshheading:11587855-Sequence Analysis, DNA, pubmed-meshheading:11587855-Sequence Homology, Amino Acid, pubmed-meshheading:11587855-Transcription, Genetic, pubmed-meshheading:11587855-Up-Regulation
pubmed:year
2001
pubmed:articleTitle
Expression profile of active genes in human periodontal ligament and isolation of PLAP-1, a novel SLRP family gene.
pubmed:affiliation
Department of Periodontology, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't