Source:http://linkedlifedata.com/resource/pubmed/id/11578597
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1-2
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| pubmed:dateCreated |
2001-10-1
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| pubmed:abstractText |
Previous work on peripheral sympathetic neurons indicated that a decline in sarco/endoplasmic reticulum calcium ATPase (SERCA) function occurs with advancing age. Therefore, an age-related decline in mechanisms controlling intracellular calcium homeostasis could contribute to altered neuronal function and/or degeneration. In this study we sought to extend the findings on peripheral neurons and to detect possible age-related declines in SERCA function and expression of SERCA3 in central neurons from cerebral cortex from young (6-month) and old (20-month) rats. Functional studies compared ATP-dependent 45Ca(2+)-uptake into microsomes and plasma membrane vesicles (PMVs). We and found no significant difference in 45Ca(2+)-uptake between microsomes or PMVs between young and old animals. On the other hand expression of SERCA3 mRNA in rat cerebral cortex showed a significant decline with advancing age. However, comparison of SERCA3 protein content did not reveal a corresponding decline; implying that SERCA mRNA turnover rates may be greater in the younger group. Although the present work with rat cerebral cortex does not indicate an age-related decline in SERCA function, previous work from our laboratory on sympathetic nerves and by others on the hippocampus indicate such a decline. In light of our previous and current studies, aging may affect calcium homeostatic mechanisms in central and peripheral autonomic neurons differently.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Atp2a3 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Transporting ATPases,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Isoforms,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Sarcoplasmic Reticulum...,
http://linkedlifedata.com/resource/pubmed/chemical/Thapsigargin
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| pubmed:status |
MEDLINE
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| pubmed:month |
Sep
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| pubmed:issn |
0006-8993
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
28
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| pubmed:volume |
914
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
57-65
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| pubmed:dateRevised |
2010-11-18
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| pubmed:meshHeading |
pubmed-meshheading:11578597-Aging,
pubmed-meshheading:11578597-Animals,
pubmed-meshheading:11578597-Calcium,
pubmed-meshheading:11578597-Calcium-Transporting ATPases,
pubmed-meshheading:11578597-Cell Membrane,
pubmed-meshheading:11578597-Cerebral Cortex,
pubmed-meshheading:11578597-Enzyme Inhibitors,
pubmed-meshheading:11578597-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:11578597-Homeostasis,
pubmed-meshheading:11578597-Intracellular Fluid,
pubmed-meshheading:11578597-Male,
pubmed-meshheading:11578597-Microsomes,
pubmed-meshheading:11578597-Nerve Degeneration,
pubmed-meshheading:11578597-Neurons,
pubmed-meshheading:11578597-Protein Isoforms,
pubmed-meshheading:11578597-RNA, Messenger,
pubmed-meshheading:11578597-Rats,
pubmed-meshheading:11578597-Rats, Inbred F344,
pubmed-meshheading:11578597-Sarcoplasmic Reticulum Calcium-Transporting ATPases,
pubmed-meshheading:11578597-Subcellular Fractions,
pubmed-meshheading:11578597-Thapsigargin,
pubmed-meshheading:11578597-Transport Vesicles
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| pubmed:year |
2001
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| pubmed:articleTitle |
Function of SERCA mediated calcium uptake and expression of SERCA3 in cerebral cortex from young and old rats.
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| pubmed:affiliation |
Department of Pharmacology, Loma Linda University School of Medicine, Loma Linda, CA 92350, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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