Linked Life Data

11577687

Source:http://linkedlifedata.com/resource/pubmed/id/11577687

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 3
pubmed:dateCreated
2001-9-28
pubmed:abstractText
A new data treatment is described for designing kinetic experiments and analysing kinetic results for multi-substrate enzymes. Normalized velocities are plotted against normalized substrate concentrations. Data are grouped into n + 1 families across the range of substrate or product tested, n being the number of substrates plus products assayed. It has the following advantages over traditional methods: (1) it reduces to less than a half the amount of data necessary for a proper description of the system; (2) it introduces a self-consistency checking parameter that ensures the 'scientific reliability' of the mathematical output; (3) it eliminates the need for a prior knowledge of Vmax; (4) the normalization of data allows the use of robust and fuzzy methods suitable for managing really 'noisy' data; (5) it is appropriate for analysing complex systems, as the complete general equation is used, and the actual influence of effectors can be typified; (6) it is amenable to being implemented as a software that incorporates testing and electing among rival kinetic models.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11577687-10909070, http://linkedlifedata.com/resource/pubmed/commentcorrection/11577687-10909071, http://linkedlifedata.com/resource/pubmed/commentcorrection/11577687-11577688, http://linkedlifedata.com/resource/pubmed/commentcorrection/11577687-1261694, http://linkedlifedata.com/resource/pubmed/commentcorrection/11577687-1584035, http://linkedlifedata.com/resource/pubmed/commentcorrection/11577687-1584042, http://linkedlifedata.com/resource/pubmed/commentcorrection/11577687-1584053, http://linkedlifedata.com/resource/pubmed/commentcorrection/11577687-1584055, http://linkedlifedata.com/resource/pubmed/commentcorrection/11577687-502857, http://linkedlifedata.com/resource/pubmed/commentcorrection/11577687-7176922, http://linkedlifedata.com/resource/pubmed/commentcorrection/11577687-7487960
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
358
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
573-83
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
A normalized plot as a novel and time-saving tool in complex enzyme kinetic analysis.
pubmed:affiliation
Departamento de Bioquímica y Biología Molecular, Universidad de León, Campus Vegazana, Spain.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't