| pubmed-article:11571581 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11571581 | lifeskim:mentions | umls-concept:C0006434 | lld:lifeskim |
| pubmed-article:11571581 | lifeskim:mentions | umls-concept:C0043250 | lld:lifeskim |
| pubmed-article:11571581 | lifeskim:mentions | umls-concept:C0023828 | lld:lifeskim |
| pubmed-article:11571581 | lifeskim:mentions | umls-concept:C0003209 | lld:lifeskim |
| pubmed-article:11571581 | lifeskim:mentions | umls-concept:C0079189 | lld:lifeskim |
| pubmed-article:11571581 | lifeskim:mentions | umls-concept:C0021665 | lld:lifeskim |
| pubmed-article:11571581 | lifeskim:mentions | umls-concept:C1515670 | lld:lifeskim |
| pubmed-article:11571581 | lifeskim:mentions | umls-concept:C1515999 | lld:lifeskim |
| pubmed-article:11571581 | lifeskim:mentions | umls-concept:C1517499 | lld:lifeskim |
| pubmed-article:11571581 | pubmed:issue | 18 | lld:pubmed |
| pubmed-article:11571581 | pubmed:dateCreated | 2001-9-25 | lld:pubmed |
| pubmed-article:11571581 | pubmed:abstractText | The use of systemic IGF-1 has been shown to attenuate the postburn hypermetabolic response and improve burn wound healing. Local IGF-1 gene therapy, however, promotes re-epithelialization in the burn wound without the side-effects associated with systemic delivery. We tested the hypothesis that these beneficial effects are due to changes in local cytokine production. Adult male Sprague-Dawley rats received a 40% total body surface area full-thickness scald burn and randomly received a subcutaneous injection at the burn wound margin of saline or cationic liposomes containing a IGF-1 cDNA construct. Animals were killed at 1, 4, 7 and 10 days after burn trauma. Skin biopsies at the wound border were harvested for total RNA extraction. Cytokine mRNA expression was determined using a multi-probe RNase protection assay. Data are presented as means +/- s.e.m. Statistical analysis used the unpaired t-test or Mann-Whitney test where appropriate. Significance was accepted at P < 0.05. Treatment of the burn wound with liposomal IGF-1-cDNA transfer decreased IL-1beta mRNA levels on day 10 after burn trauma from five-fold burn-induced increases compared with sham-treated rats, to near the control values present in unburned skin samples. Similarly, there was an eight-fold increase in TNF-alpha mRNA expression on postburn day 10 that was abrogated by IGF-1 gene therapy. Local IGF-1 gene transfer attenuates the mRNA expression of the inflammatory cytokines IL-1beta and TNF-alpha in the burn wound. This change may improve burn wound healing by decreasing prolonged local inflammation. | lld:pubmed |
| pubmed-article:11571581 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11571581 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11571581 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11571581 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11571581 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11571581 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11571581 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11571581 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11571581 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11571581 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11571581 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:11571581 | pubmed:issn | 0969-7128 | lld:pubmed |
| pubmed-article:11571581 | pubmed:author | pubmed-author:Perez-PoloJ... | lld:pubmed |
| pubmed-article:11571581 | pubmed:author | pubmed-author:SpierNN | lld:pubmed |
| pubmed-article:11571581 | pubmed:author | pubmed-author:HerndonD NDN | lld:pubmed |
| pubmed-article:11571581 | pubmed:author | pubmed-author:BarrowR ERE | lld:pubmed |
| pubmed-article:11571581 | pubmed:author | pubmed-author:NesicOO | lld:pubmed |
| pubmed-article:11571581 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11571581 | pubmed:volume | 8 | lld:pubmed |
| pubmed-article:11571581 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11571581 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11571581 | pubmed:pagination | 1409-15 | lld:pubmed |
| pubmed-article:11571581 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:11571581 | pubmed:meshHeading | pubmed-meshheading:11571581... | lld:pubmed |
| pubmed-article:11571581 | pubmed:meshHeading | pubmed-meshheading:11571581... | lld:pubmed |
| pubmed-article:11571581 | pubmed:meshHeading | pubmed-meshheading:11571581... | lld:pubmed |
| pubmed-article:11571581 | pubmed:meshHeading | pubmed-meshheading:11571581... | lld:pubmed |
| pubmed-article:11571581 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11571581 | pubmed:articleTitle | Liposomal IGF-1 gene transfer modulates pro- and anti-inflammatory cytokine mRNA expression in the burn wound. | lld:pubmed |
| pubmed-article:11571581 | pubmed:affiliation | Department of Surgery, Shriners Hospitals for Children, Galveston, TX 77550, USA. | lld:pubmed |
| pubmed-article:11571581 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11571581 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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