Source:http://linkedlifedata.com/resource/pubmed/id/11571581
Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
18
|
pubmed:dateCreated |
2001-9-25
|
pubmed:abstractText |
The use of systemic IGF-1 has been shown to attenuate the postburn hypermetabolic response and improve burn wound healing. Local IGF-1 gene therapy, however, promotes re-epithelialization in the burn wound without the side-effects associated with systemic delivery. We tested the hypothesis that these beneficial effects are due to changes in local cytokine production. Adult male Sprague-Dawley rats received a 40% total body surface area full-thickness scald burn and randomly received a subcutaneous injection at the burn wound margin of saline or cationic liposomes containing a IGF-1 cDNA construct. Animals were killed at 1, 4, 7 and 10 days after burn trauma. Skin biopsies at the wound border were harvested for total RNA extraction. Cytokine mRNA expression was determined using a multi-probe RNase protection assay. Data are presented as means +/- s.e.m. Statistical analysis used the unpaired t-test or Mann-Whitney test where appropriate. Significance was accepted at P < 0.05. Treatment of the burn wound with liposomal IGF-1-cDNA transfer decreased IL-1beta mRNA levels on day 10 after burn trauma from five-fold burn-induced increases compared with sham-treated rats, to near the control values present in unburned skin samples. Similarly, there was an eight-fold increase in TNF-alpha mRNA expression on postburn day 10 that was abrogated by IGF-1 gene therapy. Local IGF-1 gene transfer attenuates the mRNA expression of the inflammatory cytokines IL-1beta and TNF-alpha in the burn wound. This change may improve burn wound healing by decreasing prolonged local inflammation.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin-Like Growth Factor I,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Liposomes,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
|
pubmed:status |
MEDLINE
|
pubmed:month |
Sep
|
pubmed:issn |
0969-7128
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
8
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
1409-15
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:11571581-Animals,
pubmed-meshheading:11571581-Burns,
pubmed-meshheading:11571581-Cytokines,
pubmed-meshheading:11571581-Gene Expression,
pubmed-meshheading:11571581-Gene Therapy,
pubmed-meshheading:11571581-Injections, Subcutaneous,
pubmed-meshheading:11571581-Insulin-Like Growth Factor I,
pubmed-meshheading:11571581-Interleukin-1,
pubmed-meshheading:11571581-Liposomes,
pubmed-meshheading:11571581-Male,
pubmed-meshheading:11571581-RNA, Messenger,
pubmed-meshheading:11571581-Random Allocation,
pubmed-meshheading:11571581-Rats,
pubmed-meshheading:11571581-Rats, Sprague-Dawley,
pubmed-meshheading:11571581-Statistics, Nonparametric,
pubmed-meshheading:11571581-Tumor Necrosis Factor-alpha
|
pubmed:year |
2001
|
pubmed:articleTitle |
Liposomal IGF-1 gene transfer modulates pro- and anti-inflammatory cytokine mRNA expression in the burn wound.
|
pubmed:affiliation |
Department of Surgery, Shriners Hospitals for Children, Galveston, TX 77550, USA.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|