Source:http://linkedlifedata.com/resource/pubmed/id/11568169
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2001-9-24
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| pubmed:abstractText |
The effects of estrogen and ovariectomy on indexes of muscle damage after 2 h of complete hindlimb ischemia and 2 h of reperfusion were investigated in female Sprague-Dawley rats. The rats were assigned to one of three experimental groups: ovariectomized with a 17beta-estradiol pellet implant (OE), ovariectomized with a placebo pellet implant (OP), or control with intact ovaries (R). It was hypothesized that following ischemia-reperfusion (I/R), muscle damage indexes [serum creatine kinase (CK) activity, calpain-like activity, inflammatory cell infiltration, and markers of lipid peroxidation (thiobarbituric-reactive substances)] would be lower in the OE and R rats compared with the OP rats due to the protective effects of estrogen. Serum CK activity following I/R was greater (P < 0.01) in the R rats vs. OP rats and similar in the OP and OE rats. Calpain-like activity was greatest in the R rats (P < 0.01) and similar in the OP and OE rats. Neutrophil infiltration was assessed using the myeloperoxidase (MPO) assay and immunohistochemical staining for CD43-positive (CD43+) cells. MPO activity was lower (P < 0.05) in the OE rats compared with any other group and similar in the OP and R rats. The number of CD43+ cells was greater (P < 0.01) in the OP rats compared with the OE and R rats and similar in the OE and R rats. The OE rats had lower (P < 0.05) thiobarbituric-reactive substance content following I/R compared with the R and OP rats. Indexes of muscle damage were consistently attenuated in the OE rats but not in the R rats. A 10-fold difference in serum estrogen content may mediate this. Surprisingly, serum CK activity and muscle calpain-like activity were lower (P < 0.05) in the OP rats compared with the R rats. Increases in serum insulin-like growth factor-1 content (P < 0.05) due to ovariectomy were hypothesized to account for this finding. Thus both ovariectomy and estrogen supplementation have differential effects on indexes of I/R muscle damage.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD43,
http://linkedlifedata.com/resource/pubmed/chemical/Calpain,
http://linkedlifedata.com/resource/pubmed/chemical/Creatine Kinase,
http://linkedlifedata.com/resource/pubmed/chemical/Estrogens,
http://linkedlifedata.com/resource/pubmed/chemical/Glycogen,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin-Like Growth Factor I,
http://linkedlifedata.com/resource/pubmed/chemical/Lactic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Peroxidase,
http://linkedlifedata.com/resource/pubmed/chemical/Sialoglycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Spn protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Thiobarbituric Acid Reactive...
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| pubmed:status |
MEDLINE
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| pubmed:month |
Oct
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| pubmed:issn |
8750-7587
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
91
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1828-35
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:11568169-Animals,
pubmed-meshheading:11568169-Antigens, CD,
pubmed-meshheading:11568169-Antigens, CD43,
pubmed-meshheading:11568169-Calpain,
pubmed-meshheading:11568169-Creatine Kinase,
pubmed-meshheading:11568169-Estrogens,
pubmed-meshheading:11568169-Female,
pubmed-meshheading:11568169-Glycogen,
pubmed-meshheading:11568169-Hindlimb,
pubmed-meshheading:11568169-Insulin-Like Growth Factor I,
pubmed-meshheading:11568169-Lactic Acid,
pubmed-meshheading:11568169-Lipid Peroxidation,
pubmed-meshheading:11568169-Muscle, Skeletal,
pubmed-meshheading:11568169-Neutrophil Infiltration,
pubmed-meshheading:11568169-Ovariectomy,
pubmed-meshheading:11568169-Peroxidase,
pubmed-meshheading:11568169-Rats,
pubmed-meshheading:11568169-Rats, Sprague-Dawley,
pubmed-meshheading:11568169-Reperfusion Injury,
pubmed-meshheading:11568169-Sialoglycoproteins,
pubmed-meshheading:11568169-Thiobarbituric Acid Reactive Substances
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| pubmed:year |
2001
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| pubmed:articleTitle |
Effects of ovariectomy and estrogen on ischemia-reperfusion injury in hindlimbs of female rats.
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| pubmed:affiliation |
Department of Kinesiology and Physical Education, Wilfrid Laurier University, Waterloo, Ontario, Canada N2L 3C5.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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