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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2001-9-21
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pubmed:abstractText |
Angiotensin (Ang) II has 2 major receptor isoforms, Ang type 1 (AT(1)) and Ang type (AT(2)). AT(1) transphosphorylates epidermal growth factor receptor (EGFR) to activate extracellular signal-regulated kinase (ERK). Although AT(2) was shown to inactivate ERK, the action of AT(2) on EGFR activation remains undefined. Using AT(2)-overexpressing vascular smooth muscle cells from AT(2) transgenic mice, we studied these undefined actions of AT(2). Maximal ERK activity induced by Ang II was increased 1.9- and 2.2-fold by AT(2) inhibition, which was abolished by orthovanadate but not okadaic acid or pertussis toxin. AT(2) inhibited AT(1)-mediated EGFR tyrosine phosphorylation by 63%. The activity of SHP-1 tyrosine phosphatase was significantly upregulated 1 minute after AT(2) stimulation and association of SHP-1 with EGFR was increased, whereas AT(2) failed to tyrosine phosphorylate SHP-1. Stable overexpression of SHP-1-dominant negative mutant completely abolished AT(2)-mediated inhibition of EGFR and ERK activation. AT(1)-mediated c-fos mRNA accumulation was attenuated by 48% by AT(2) stimulation. Induction of fibronectin gene containing an AP-1 responsive element in its 5'-flanking region was decreased by 37% after AT(2) stimulation, corresponding to the results of gel mobility assay with the AP-1 sequence of fibronectin as a probe. These findings suggested that AT(2) inhibits ERK activity by inducing SHP-1 activity, leading to decreases in AP-1 activity and AP-1-regulated gene expression, in which EGFR dephosphorylation plays an important role via association of SHP-1.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Angiotensin II,
http://linkedlifedata.com/resource/pubmed/chemical/Fibronectins,
http://linkedlifedata.com/resource/pubmed/chemical/Imidazoles,
http://linkedlifedata.com/resource/pubmed/chemical/Intracellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/PD 123319,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphotyrosine,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Tyrosine Phosphatase...,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Tyrosine Phosphatase...,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Tyrosine Phosphatase...,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Tyrosine Phosphatases,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-fos,
http://linkedlifedata.com/resource/pubmed/chemical/Ptpn11 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Ptpn6 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Pyridines,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Angiotensin, Type 1,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Angiotensin, Type 2,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Epidermal Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Angiotensin,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor AP-1
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1524-4563
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pubmed:author |
pubmed-author:FujiyamaSS,
pubmed-author:HasegawaTT,
pubmed-author:HoriuchiMM,
pubmed-author:IbeKK,
pubmed-author:IwasakaTT,
pubmed-author:KosakaYY,
pubmed-author:MOEDH KHK,
pubmed-author:MasakiHH,
pubmed-author:MatsubaraHH,
pubmed-author:MoriYY,
pubmed-author:NahmiasCC,
pubmed-author:NozawaYY,
pubmed-author:ShibasakiYY,
pubmed-author:TateishiEE,
pubmed-author:TsutsumiYY,
pubmed-author:UchiyamaYY
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pubmed:issnType |
Electronic
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pubmed:volume |
38
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
367-72
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:11566906-Angiotensin II,
pubmed-meshheading:11566906-Animals,
pubmed-meshheading:11566906-Cells, Cultured,
pubmed-meshheading:11566906-Fibronectins,
pubmed-meshheading:11566906-Gene Expression Regulation,
pubmed-meshheading:11566906-Imidazoles,
pubmed-meshheading:11566906-Intracellular Signaling Peptides and Proteins,
pubmed-meshheading:11566906-Mice,
pubmed-meshheading:11566906-Mice, Transgenic,
pubmed-meshheading:11566906-Mitogen-Activated Protein Kinases,
pubmed-meshheading:11566906-Muscle, Smooth, Vascular,
pubmed-meshheading:11566906-Phosphorylation,
pubmed-meshheading:11566906-Phosphotyrosine,
pubmed-meshheading:11566906-Protein Binding,
pubmed-meshheading:11566906-Protein Tyrosine Phosphatase, Non-Receptor Type 1,
pubmed-meshheading:11566906-Protein Tyrosine Phosphatase, Non-Receptor Type 11,
pubmed-meshheading:11566906-Protein Tyrosine Phosphatase, Non-Receptor Type 6,
pubmed-meshheading:11566906-Protein Tyrosine Phosphatases,
pubmed-meshheading:11566906-Proto-Oncogene Proteins c-fos,
pubmed-meshheading:11566906-Pyridines,
pubmed-meshheading:11566906-RNA, Messenger,
pubmed-meshheading:11566906-Receptor, Angiotensin, Type 1,
pubmed-meshheading:11566906-Receptor, Angiotensin, Type 2,
pubmed-meshheading:11566906-Receptor, Epidermal Growth Factor,
pubmed-meshheading:11566906-Receptors, Angiotensin,
pubmed-meshheading:11566906-Transcription Factor AP-1
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pubmed:year |
2001
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pubmed:articleTitle |
Angiotensin II type 2 receptor inhibits epidermal growth factor receptor transactivation by increasing association of SHP-1 tyrosine phosphatase.
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pubmed:affiliation |
Department of Medicine II, Kansai Medical University, Moriguchi, Osaka, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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