Linked Life Data

11563844

Source:http://linkedlifedata.com/resource/pubmed/id/11563844

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2001-9-20
pubmed:abstractText
Physical shock wave (SW) has shown effectiveness on promotion of bone growth. We have recently demonstrated that SW could promote bone marrow stromal cell differentiation toward osteoprogenitor associated with induction of TGF-beta1. We have further demonstrated that SW-induced membrane hyperpolarization and Ras activation acted an early signal for the osteogenesis in human bone marrow stromal cells. An optimal dose of SW treatment at 0.16 mJ/mm(2) for 500 impulses induced a rapid membrane hyperpolarization in 5 min, activation of Ras in 30 min, and cell proliferation in 2 days. The SW-promoted cell growth was related to osteogenesis as demonstrated by increase of bone alkaline phosphatase activity in 6 days and osteocalcin mRNA expression in 12 days. In support that SW-induced Ras activation mediated osteogenesis of human bone marrow stromal cells, we further demonstrated that transfection of bone marrow stromal cells with a dominant negative Ras mutant (Asn-17 ras(H)) abrogated the SW enhancement of osteogenic transcription factor (CBFA1) activation, osteocalcin mRNA expression, and bone nodule formations. These results suggest that physical SW promotes bone marrow stromal cell differentiation toward osteogenic lineage via membrane hyperpolarization, followed by Ras activation and specific osteogenic transcription factor CBFA1 expression. A link between physical SW and biomembrane perturbation-mediated Ras activation may highlight how noninvasive physical agents could be used to promote fracture healing and to rescue patients with osteoporosis and osteopenic disorders in the future.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0006-291X
pubmed:author
pubmed:copyrightInfo
Copyright 2001 Academic Press.
pubmed:issnType
Print
pubmed:day
28
pubmed:volume
287
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
648-55
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11563844-Bone Marrow Cells, pubmed-meshheading:11563844-Cell Division, pubmed-meshheading:11563844-Cell Line, pubmed-meshheading:11563844-Cell Membrane, pubmed-meshheading:11563844-Cell Survival, pubmed-meshheading:11563844-Cells, Cultured, pubmed-meshheading:11563844-Core Binding Factor Alpha 1 Subunit, pubmed-meshheading:11563844-Enzyme Activation, pubmed-meshheading:11563844-Genes, Dominant, pubmed-meshheading:11563844-High-Energy Shock Waves, pubmed-meshheading:11563844-Humans, pubmed-meshheading:11563844-Kinetics, pubmed-meshheading:11563844-Membrane Potentials, pubmed-meshheading:11563844-Mutation, pubmed-meshheading:11563844-Neoplasm Proteins, pubmed-meshheading:11563844-Osteogenesis, pubmed-meshheading:11563844-RNA, Messenger, pubmed-meshheading:11563844-Signal Transduction, pubmed-meshheading:11563844-Time Factors, pubmed-meshheading:11563844-Transcription Factors, pubmed-meshheading:11563844-Transfection, pubmed-meshheading:11563844-Transforming Growth Factor beta, pubmed-meshheading:11563844-Transforming Growth Factor beta1, pubmed-meshheading:11563844-ras Proteins
pubmed:year
2001
pubmed:articleTitle
Physical shock wave mediates membrane hyperpolarization and Ras activation for osteogenesis in human bone marrow stromal cells.
pubmed:affiliation
Department of Medical Research, Chang Gung University, Kaohsiung, Taiwan, Republic of China.
pubmed:publicationType
Journal Article