Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2001-9-20
pubmed:abstractText
Stainless steel and ceramic surfaces were subjected to repeated soiling and cleaning procedures, using a milk powder soil inoculated with Pseudomonas aeruginosa and Staphylococcus aureus, followed by spraying with water with or without 0.1% (vol/vol) nonionic detergent. Test surfaces were removed after 1, 5, 10, and 20 soiling-cleaning cycles and were analyzed for attached microorganisms (total viable count of bacteria removed by swabbing and percentage of coverage of cells plus soil stained with acridine orange). Surfaces were additionally examined using a range of analytical techniques: X-ray photoelectron spectroscopy (XPS), dynamic and imaging secondary ion mass spectroscopy (SIMS), and time-of-flight SIMS. Both microbiological and chemical analyses revealed an accumulation of material over the cycles to a maximum level. Surfaces were conditioned rapidly to saturation with organic material within one cycle (XPS), whereas fouling by microorganisms was less rapid (five cycles). Cleaning with detergent tended to retard the cumulative fouling process when compared with spray cleaning using water alone. The methods described provide a more realistic scenario for testing the cleanability of surfaces routinely found in food processing locations and for screening novel cleaning regimens and/or surface materials. The analytical techniques provide additional information on the kinetics of soiling, which complement the more familiar microbiological methods.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0362-028X
pubmed:author
pubmed:issnType
Print
pubmed:volume
64
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1377-87
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
Microbiological and chemical analyses of stainless steel and ceramics subjected to repeated soiling and cleaning treatments.
pubmed:affiliation
Department of Biological Sciences, Manchester Metropolitan University, England. j.verran@mmu.ac.uk
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't