Source:http://linkedlifedata.com/resource/pubmed/id/11562149
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
2001-9-19
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| pubmed:abstractText |
C-C chemokines are soluble mediators that occur in a periprosthetic granuloma and influence recruitment, localization and activation of inflammatory cells. This study tested effects of titanium and polymethylmethacrylate (PMMA) particles on expression of selected C-C chemokines in cultured human fibroblasts. The C-C chemokines analyzed included monocyte chemoattractant protein-1. 2 (MCP-1. 2), monocyte inflammatory protein-1 alpha (MIP-1 alpha), and regulated on activation, normal T-cell expressed and secreted protein (RANTES). Interleukin-1 beta (IL-1 beta) served as a known stimulator of chemokine release while interleukin-6 (IL-6) expression served as a marker for fibroblast activation. Protein and mRNA signal levels were determined by ELISA and RT-PCR, respectively. The results demonstrated that exposure of fibroblasts to titanium and PMMA particles resulted in increased release of MCP-1 in a dose- and time-dependent manner. After 24 h, titanium particles maximally upregulated MCP-1 release 7-fold while PMMA particles increased MCP-1 levels 2-fold, when compared to unchallenged fibroblasts. MCP-2, MIP-1 alpha and RANTES levels remained unchanged following exposure of fibroblasts to titanium or PMMA particles at any concentration or time point tested. However, IL-1 beta stimulated release of MCP-1, MCP-2, and RANTES, but not MIP-1 alpha from the fibroblasts. IL-1 beta, not particles, exhibited the most prominent effect on MCP-1 mRNA levels. Increased release of MCP-1 from fibroblasts exposed to titanium and PMMA particles coincided with increased release of IL-6. This study suggests that release of chemoattractant factors from fibroblasts localized in periprosthetic membranes enhances the chronic inflammatory process leading to bone resorption and implant loosening.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bone Cements,
http://linkedlifedata.com/resource/pubmed/chemical/CCL8 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CCL2,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CCL4,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CCL5,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CCL8,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokines, CC,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-6,
http://linkedlifedata.com/resource/pubmed/chemical/Macrophage Inflammatory Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Monocyte Chemoattractant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Polymethyl Methacrylate,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Titanium
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0736-0266
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
19
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
970-6
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| pubmed:dateRevised |
2007-11-15
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| pubmed:meshHeading |
pubmed-meshheading:11562149-Bone Cements,
pubmed-meshheading:11562149-Cells, Cultured,
pubmed-meshheading:11562149-Chemokine CCL2,
pubmed-meshheading:11562149-Chemokine CCL4,
pubmed-meshheading:11562149-Chemokine CCL5,
pubmed-meshheading:11562149-Chemokine CCL8,
pubmed-meshheading:11562149-Chemokines, CC,
pubmed-meshheading:11562149-Fibroblasts,
pubmed-meshheading:11562149-Gene Expression,
pubmed-meshheading:11562149-Humans,
pubmed-meshheading:11562149-Interleukin-1,
pubmed-meshheading:11562149-Interleukin-6,
pubmed-meshheading:11562149-Macrophage Inflammatory Proteins,
pubmed-meshheading:11562149-Materials Testing,
pubmed-meshheading:11562149-Monocyte Chemoattractant Proteins,
pubmed-meshheading:11562149-Polymethyl Methacrylate,
pubmed-meshheading:11562149-RNA, Messenger,
pubmed-meshheading:11562149-Skin,
pubmed-meshheading:11562149-T-Lymphocytes,
pubmed-meshheading:11562149-Titanium
|
| pubmed:year |
2001
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| pubmed:articleTitle |
Fibroblast expression of C-C chemokines in response to orthopaedic biomaterial particle challenge in vitro.
|
| pubmed:affiliation |
Orthopaedic Research Laboratory, Stanford University, School of Medicine, CA 94305-5341, USA.
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| pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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