Linked Life Data

11559894

Source:http://linkedlifedata.com/resource/pubmed/id/11559894

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2001-9-17
pubmed:abstractText
Nerve growth factor (NGF) and related neurotrophins induce differential axon growth patterns from embryonic sensory neurons (Lentz et al. [1999] J. Neurosci. 19:1038-1048; Ulupinar et al. [2000a] J. Comp. Neurol 425:622-630). In wholemount explant cultures of embryonic rat trigeminal ganglion and brainstem or in dissociated cell cultures of the trigeminal ganglion, exogenous supply of NGF leads to axonal elongation, whereas neurotrophin-3 (NT-3) treatment leads to short branching and arborization (Ulupinar et al. [2000a] J. Comp. Neurol. 425:622-630). Axonal responses to neurotrophins might be mediated via the Rho GTPases. To investigate this possibility, we prepared wholemount trigeminal pathway cultures from E15 rats. We infected the ganglia with recombinant vaccinia viruses that express GFP-tagged dominant negative Rac, Rho, or constitutively active Rac or treated the cultures with lysophosphatitic acid (LPA) to activate Rho. We then examined axonal responses to NGF by use of the lipophilic tracer DiI. Rac activity induced longer axonal growth from the central trigeminal tract, whereas the dominant negative construct of Rac eliminated NGF-induced axon outgrowth. Rho activity also significantly reduced, and the Rho dominant negative construct increased, axon growth from the trigeminal tract. Similar alterations in axonal responses to NT-3 and brain-derived neurotrophic factor were also noted. Our results demonstrate that Rho GTPases play a major role in neurotrophin-induced axonal differentiation of embryonic trigeminal axons.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0021-9967
pubmed:author
pubmed:copyrightInfo
Copyright 2001 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
438
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
377-87
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:11559894-Afferent Pathways, pubmed-meshheading:11559894-Animals, pubmed-meshheading:11559894-Brain-Derived Neurotrophic Factor, pubmed-meshheading:11559894-Carbocyanines, pubmed-meshheading:11559894-Cell Differentiation, pubmed-meshheading:11559894-Cell Size, pubmed-meshheading:11559894-Fetus, pubmed-meshheading:11559894-Fluorescent Dyes, pubmed-meshheading:11559894-Gene Expression Regulation, Developmental, pubmed-meshheading:11559894-Genetic Vectors, pubmed-meshheading:11559894-Growth Cones, pubmed-meshheading:11559894-Immunohistochemistry, pubmed-meshheading:11559894-Lysophospholipids, pubmed-meshheading:11559894-Nerve Growth Factor, pubmed-meshheading:11559894-Nerve Growth Factors, pubmed-meshheading:11559894-Neurons, Afferent, pubmed-meshheading:11559894-Neurotrophin 3, pubmed-meshheading:11559894-Rats, pubmed-meshheading:11559894-Rats, Sprague-Dawley, pubmed-meshheading:11559894-Transfection, pubmed-meshheading:11559894-Trigeminal Ganglion, pubmed-meshheading:11559894-Trigeminal Nuclei, pubmed-meshheading:11559894-Vaccinia virus, pubmed-meshheading:11559894-rac GTP-Binding Proteins, pubmed-meshheading:11559894-rho GTP-Binding Proteins
pubmed:year
2001
pubmed:articleTitle
Regulation of neurotrophin-induced axonal responses via Rho GTPases.
pubmed:affiliation
Department of Cell Biology and Anatomy, LSUHSC, New Orleans, Louisiana 70112, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.