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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
13
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pubmed:dateCreated |
2001-9-17
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pubmed:abstractText |
Evaluation of immune mechanisms responsible for control of viral replication is critical to understanding HIV-2 attenuated biological characteristics in pathogenesis and transmission. Evaluation of the cellular immune response is often based on labor-intensive techniques that limit the scope of most studies performed. A simple and rapid anthrax toxin-based ELISPOT method to assess HIV-2 cellular immune response was developed. The modified anthrax toxin-based antigen presentation process performed better than a recombinant vaccinia system and the ELISPOT method significantly enhanced the ease and simplicity of the assay. Using this method, a robust HIV-2 cellular immune response directed toward the p26 core protein was exhibited in 21 of 24 (87.5%) infected women, and all 8 seronegative subjects were negative in both assays. Cellular immune responses were associated with low HIV-2 viral load. This simple and rapid modified anthrax toxin-based ELISPOT method allowed us to demonstrate, strong cellular immune responses that may be critical determinants in the HIV-2 attenuated phenotype.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Toxins,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, gag,
http://linkedlifedata.com/resource/pubmed/chemical/HIV Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/anthrax toxin,
http://linkedlifedata.com/resource/pubmed/chemical/gag Gene Products, Human...,
http://linkedlifedata.com/resource/pubmed/chemical/gag protein p26, Human...
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0889-2229
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
17
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1257-64
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:11559425-Antigen Presentation,
pubmed-meshheading:11559425-Antigens, Bacterial,
pubmed-meshheading:11559425-Bacterial Toxins,
pubmed-meshheading:11559425-Cells, Cultured,
pubmed-meshheading:11559425-Female,
pubmed-meshheading:11559425-Gene Products, gag,
pubmed-meshheading:11559425-HIV Antigens,
pubmed-meshheading:11559425-HIV Infections,
pubmed-meshheading:11559425-HIV-2,
pubmed-meshheading:11559425-Humans,
pubmed-meshheading:11559425-Immunoenzyme Techniques,
pubmed-meshheading:11559425-Leukocytes, Mononuclear,
pubmed-meshheading:11559425-Monitoring, Immunologic,
pubmed-meshheading:11559425-RNA, Viral,
pubmed-meshheading:11559425-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:11559425-T-Lymphocytes, Helper-Inducer,
pubmed-meshheading:11559425-Viral Load,
pubmed-meshheading:11559425-gag Gene Products, Human Immunodeficiency Virus
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pubmed:year |
2001
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pubmed:articleTitle |
Robust HIV type 2 cellular immune response measured by a modified anthrax toxin-based enzyme-linked immunospot assay.
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pubmed:affiliation |
Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts 02115, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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