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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2001-9-14
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pubmed:abstractText |
Serotonin (5-HT) type 3 receptor (5-HT(3)-R) is a ligand-gated ion channel found primarily in the central and peripheral nervous system. We report expression and functional characterization of 5-HT(3)-R in pulmonary neuroepithelial body (NEB) cells. Using nonisotopic in situ hybridization, we demonstrate expression of 5-HT(3)-R mRNA in NEB cells in the lungs of different mammals (hamster, rabbit, mouse, and human). Dual immunocytochemistry (for 5-HT and 5-HT(3)-R) and confocal microscopy localized 5-HT(3)-R on NEB cell plasma membrane from rabbit. The electrophysiological characteristics of 5-HT(3)-R in NEB cells were studied in fresh slices of neonatal hamster lung using the whole cell patch-clamp technique. Application of the 5-HT (5-150 microM) and 5-HT(3)-R agonist 2-methyl-5-HT (5-150 microM) induced inward currents in a concentration-dependent manner. The 5-HT-induced current was blocked (76.5 +/- 5.9%) by the specific 5-HT(3)-R antagonist ICS-205-930 (50 microM), whereas katanserin and p-4-iodo-N-(2-[4-(methoxyphenyl)-1-piperazinyl]ethyl)-N-2-pyridinylbenzamide had minimal effects. Forskolin had no effect on desensitization and amplitude of the 5-HT-induced current. The reduction of Ca(2+) and Mg(2+) in the extracellular solution enhanced the amplitude of the 5-HT-induced current because of slower desensitization. Our studies suggest that 5-HT(3)-R in NEB cells may function as an autoreceptor and may potentially be involved in modulation of hypoxia signaling.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2-methyl-5-HT,
http://linkedlifedata.com/resource/pubmed/chemical/4-(2'-methoxyphenyl)-1-(2'-(N-(2''-p...,
http://linkedlifedata.com/resource/pubmed/chemical/Aminopyridines,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Forskolin,
http://linkedlifedata.com/resource/pubmed/chemical/Indoles,
http://linkedlifedata.com/resource/pubmed/chemical/Ketanserin,
http://linkedlifedata.com/resource/pubmed/chemical/Piperazines,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Serotonin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Serotonin, 5-HT3,
http://linkedlifedata.com/resource/pubmed/chemical/Serotonin,
http://linkedlifedata.com/resource/pubmed/chemical/Serotonin Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/tropisetron
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1040-0605
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
281
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
L931-40
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:11557597-Aminopyridines,
pubmed-meshheading:11557597-Animals,
pubmed-meshheading:11557597-Calcium,
pubmed-meshheading:11557597-Chemoreceptor Cells,
pubmed-meshheading:11557597-Cricetinae,
pubmed-meshheading:11557597-Dose-Response Relationship, Drug,
pubmed-meshheading:11557597-Forskolin,
pubmed-meshheading:11557597-Gene Expression,
pubmed-meshheading:11557597-Humans,
pubmed-meshheading:11557597-Immunohistochemistry,
pubmed-meshheading:11557597-In Situ Hybridization,
pubmed-meshheading:11557597-Indoles,
pubmed-meshheading:11557597-Ion Channel Gating,
pubmed-meshheading:11557597-Ketanserin,
pubmed-meshheading:11557597-Lung,
pubmed-meshheading:11557597-Mammals,
pubmed-meshheading:11557597-Membrane Potentials,
pubmed-meshheading:11557597-Mesocricetus,
pubmed-meshheading:11557597-Mice,
pubmed-meshheading:11557597-Mice, Inbred C57BL,
pubmed-meshheading:11557597-Patch-Clamp Techniques,
pubmed-meshheading:11557597-Piperazines,
pubmed-meshheading:11557597-RNA, Messenger,
pubmed-meshheading:11557597-Rabbits,
pubmed-meshheading:11557597-Receptors, Serotonin,
pubmed-meshheading:11557597-Receptors, Serotonin, 5-HT3,
pubmed-meshheading:11557597-Respiratory Mucosa,
pubmed-meshheading:11557597-Serotonin,
pubmed-meshheading:11557597-Serotonin Antagonists
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pubmed:year |
2001
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pubmed:articleTitle |
Neuroepithelial bodies in mammalian lung express functional serotonin type 3 receptor.
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pubmed:affiliation |
Division of Pathology, Department of Pediatric Laboratory Medicine, The Research Institute, The Hospital for Sick Children and University of Toronto, Toronto, Ontario, Canada M5G 1X8.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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