Linked Life Data

11556845

Source:http://linkedlifedata.com/resource/pubmed/id/11556845

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2001-9-14
pubmed:abstractText
The urokinase-dependent activation of plasminogen by breast cancer cells plays an important role in metastasis. We have previously shown that the metastatic breast cancer cell line MDA-MB-231 over-expresses urokinase and binds and efficiently activates plasminogen at the cell surface compared to non-metastatic cells. The aim of this study was to further characterise plasminogen binding and determine the topology of cell surface-bound plasminogen in terms of its potential for activation. The lysine-dependent binding of plasminogen at 4 degrees C to MDA-MB-231 cells was stable and resulted in an activation-susceptible conformation of plasminogen. Topologically, a fraction of bound plasminogen was co-localised with urokinase on the surfaces of MDA-MB-231 cells where it could be activated to plasmin. At 37 degrees C plasmin was rapidly lost from the cell surface. Apart from actin, other candidate plasminogen receptors were either not expressed or did not co-localise with plasminogen at the cell surface. Thus, based on co-localisation with urokinase, plasminogen binding is partitioned into two functional pools on the surface of MDA-MB-231 cells. In conclusion, these results shed further light on the functional organisation of the plasminogen activation cascade on the surface of a metastatic cancer cell.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11556845-10190288, http://linkedlifedata.com/resource/pubmed/commentcorrection/11556845-10389872, http://linkedlifedata.com/resource/pubmed/commentcorrection/11556845-1991514, http://linkedlifedata.com/resource/pubmed/commentcorrection/11556845-3023326, http://linkedlifedata.com/resource/pubmed/commentcorrection/11556845-3880760, http://linkedlifedata.com/resource/pubmed/commentcorrection/11556845-7593646, http://linkedlifedata.com/resource/pubmed/commentcorrection/11556845-7851415, http://linkedlifedata.com/resource/pubmed/commentcorrection/11556845-8239651, http://linkedlifedata.com/resource/pubmed/commentcorrection/11556845-8710884, http://linkedlifedata.com/resource/pubmed/commentcorrection/11556845-8857956, http://linkedlifedata.com/resource/pubmed/commentcorrection/11556845-9003434, http://linkedlifedata.com/resource/pubmed/commentcorrection/11556845-9083329, http://linkedlifedata.com/resource/pubmed/commentcorrection/11556845-9635833, http://linkedlifedata.com/resource/pubmed/commentcorrection/11556845-9665813, http://linkedlifedata.com/resource/pubmed/commentcorrection/11556845-9888805
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0007-0920
pubmed:author
pubmed:copyrightInfo
Copyright 2001 Cancer Research Campaign http://www.bjcancer.com.
pubmed:issnType
Print
pubmed:day
14
pubmed:volume
85
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
909-16
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
The topology of plasminogen binding and activation on the surface of human breast cancer cells.
pubmed:affiliation
Department of Biological Sciences, University of Wollongong, NSW Australia, 2522.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't