Source:http://linkedlifedata.com/resource/pubmed/id/11554969
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
Pt 5
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pubmed:dateCreated |
2001-9-13
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pubmed:abstractText |
In this paper we present the analysis of nucleolar organizer regions (NORs) in five species of the order Pleuronectiformes (Scophthalmus maximus, Scophthalmus rhombus, Platichthys flesus, Solea solea and Solea lascaris), a group of fish poorly studied cytogenetically. In spite of the small chromosome sizes, which characterize the karyotypes of this group, we implement both classical (Ag- and CMA3-staining) and molecular (in situ hybridization with a major rDNA probe) techniques for NOR location. NORs were localized in a single chromosome pair in all species studied and showed size variation within each species. Evidence of a NOR-site polymorphism was detected in Platichthys flesus and Scophthalmus maximus. NOR location was apparently the same within families (S. rhombus vs. S maximus, and S. solea vs. S. lascaris) but clearly differed among families, and supports the phylogenetic relationships proposed for the order Pleuronectiformes.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0018-067X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
86
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
531-6
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11554969-Animals,
pubmed-meshheading:11554969-Chromosome Banding,
pubmed-meshheading:11554969-Chromosomes,
pubmed-meshheading:11554969-Coloring Agents,
pubmed-meshheading:11554969-DNA, Ribosomal,
pubmed-meshheading:11554969-Flatfishes,
pubmed-meshheading:11554969-Genes, rRNA,
pubmed-meshheading:11554969-In Situ Hybridization,
pubmed-meshheading:11554969-Karyotyping,
pubmed-meshheading:11554969-Metaphase,
pubmed-meshheading:11554969-Physical Chromosome Mapping,
pubmed-meshheading:11554969-Silver Staining
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pubmed:year |
2001
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pubmed:articleTitle |
Localization of ribosomal genes in Pleuronectiformes using Ag-, CMA3-banding and in situ hybridization.
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pubmed:affiliation |
Departamento de Biología Fundamental, Area de Genética, Facultad de Veterinaria, Universidad de Santiago de Compostela, 27002 Lugo, Spain.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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