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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2001-9-13
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pubmed:abstractText |
The CXC chemokine CXCL13, known as BCA-1 (B cell-attracting chemokine 1) or BLC (B-lymphocyte chemoattractant), has been identified as an efficacious attractant selective for B lymphocytes. The chemokine receptor BLR1 (Burkitt's lymphoma receptor 1)/CXCR5 expressed by all mature B cells has to date been identified as the only known receptor for BCA-1. As the loss of the BLR1/CXCR5 receptor is sufficient to disrupt organization of follicles in spleen and Peyer's patches, BCA-1 may act as a B cell homing chemokine. Nonetheless, BCA-1 has not been tested against all known chemokine receptors. In this study, we report that human BCA-1 competes with radiolabeled interferon gamma (IFN-gamma) inducible protein 10 (IP-10) for binding to the human CXCR3 receptor expressed in Ba/F3 and 293EBNA cell lines. Furthermore, human BCA-1 is an efficacious attractant for human CXCR3 transfected cells; BCA-1-induced chemotaxis is inhibited by a monoclonal antibody against human CXCR3. In these cells, as in human B lymphocytes expressing CXCR5, BCA-1 does not induce a calcium flux. Indeed, BCA-1 attenuates the calcium flux induced by IP-10. In addition, human BCA-1 is an agonist in stimulating GTP gamma S binding. Together these data suggest that human BCA-1 is a specific and functional G-protein-linked chemotactic ligand for the human CXCR3 receptor. The biological significance of this new finding is supported by our recent observation that human BCA-1 induces chemotaxis of activated T cells and the BCA-1-induced chemotaxis is inhibited by a monoclonal antibody against human CXCR3.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Blr1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/CXCL13 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/CXCR3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/CXCR5 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CXCL10,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CXCL13,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokines,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokines, CXC,
http://linkedlifedata.com/resource/pubmed/chemical/Cxcl13 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Cxcr3 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Cxcr3 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Guanosine 5'-O-(3-Thiotriphosphate),
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Ligands,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, CXCR3,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, CXCR5,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Chemokine,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cytokine
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1043-4666
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2001 Academic Press.
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pubmed:issnType |
Print
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pubmed:day |
7
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pubmed:volume |
15
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
113-21
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:11554781-Animals,
pubmed-meshheading:11554781-Antibodies, Monoclonal,
pubmed-meshheading:11554781-B-Lymphocytes,
pubmed-meshheading:11554781-Calcium,
pubmed-meshheading:11554781-Cell Line,
pubmed-meshheading:11554781-Cell Membrane,
pubmed-meshheading:11554781-Cell Separation,
pubmed-meshheading:11554781-Chemokine CXCL10,
pubmed-meshheading:11554781-Chemokine CXCL13,
pubmed-meshheading:11554781-Chemokines,
pubmed-meshheading:11554781-Chemokines, CXC,
pubmed-meshheading:11554781-Chemotaxis,
pubmed-meshheading:11554781-DNA, Complementary,
pubmed-meshheading:11554781-Dose-Response Relationship, Drug,
pubmed-meshheading:11554781-Flow Cytometry,
pubmed-meshheading:11554781-Guanosine 5'-O-(3-Thiotriphosphate),
pubmed-meshheading:11554781-Humans,
pubmed-meshheading:11554781-Interferon-gamma,
pubmed-meshheading:11554781-Ligands,
pubmed-meshheading:11554781-Mice,
pubmed-meshheading:11554781-Protein Binding,
pubmed-meshheading:11554781-Rats,
pubmed-meshheading:11554781-Receptors, CXCR3,
pubmed-meshheading:11554781-Receptors, CXCR5,
pubmed-meshheading:11554781-Receptors, Chemokine,
pubmed-meshheading:11554781-Receptors, Cytokine,
pubmed-meshheading:11554781-Time Factors,
pubmed-meshheading:11554781-Transfection
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pubmed:year |
2001
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pubmed:articleTitle |
Human B cell-attracting chemokine 1 (BCA-1; CXCL13) is an agonist for the human CXCR3 receptor.
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pubmed:affiliation |
Department of Immunology, Schering-Plough Research Institute, Kenilworth, NJ 07033, USA. Chung-Her.Jenh@spcorp.com
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pubmed:publicationType |
Journal Article
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