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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2001-9-13
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pubmed:abstractText |
Reactive oxygen species have been shown to play important roles in v-Ha-Ras mitogenic signaling. We hypothesized that v-Ha-Ras overexpression would induce superoxide production, and therefore modify expression of the primary antioxidant enzyme system. We have demonstrated that immortal rat kidney epithelial cells stably transduced with constitutively active v-Ha-ras produced significantly larger amounts of superoxide radical than wild-type or vector-transfected control cells. The levels of the primary antioxidant enzymes copper- and zinc-containing superoxide dismutase, manganese-containing superoxide dismutase, catalase, and glutathione peroxidase were increased in the superoxide-overproducing cells. DNA-binding activities of the transcription factors activator protein-1, activator protein-2, and nuclear factor-kappaB were all enhanced in the superoxide-overproducing cells. These v-Ha-ras transduced cells also had a shortened cell doubling time and higher plating efficiency, and displayed greater constitutive levels of phosphorylated mitogen-activated protein kinases. These data demonstrate that v-Ha-Ras overexpression increases superoxide production and this apparently affects a wide variety of cell signaling and redox systems.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antioxidants,
http://linkedlifedata.com/resource/pubmed/chemical/Catalase,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Glutathione Peroxidase,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Oncogene Protein p21(ras),
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Superoxide Dismutase,
http://linkedlifedata.com/resource/pubmed/chemical/Superoxides,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor AP-1,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor AP-2,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1523-0864
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
3
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
697-709
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:11554455-Animals,
pubmed-meshheading:11554455-Antioxidants,
pubmed-meshheading:11554455-Catalase,
pubmed-meshheading:11554455-Cell Division,
pubmed-meshheading:11554455-Cell Line, Transformed,
pubmed-meshheading:11554455-Cell Transformation, Neoplastic,
pubmed-meshheading:11554455-DNA,
pubmed-meshheading:11554455-DNA-Binding Proteins,
pubmed-meshheading:11554455-Electrophoretic Mobility Shift Assay,
pubmed-meshheading:11554455-Epithelial Cells,
pubmed-meshheading:11554455-Glutathione Peroxidase,
pubmed-meshheading:11554455-Kidney,
pubmed-meshheading:11554455-MAP Kinase Signaling System,
pubmed-meshheading:11554455-Mitogen-Activated Protein Kinases,
pubmed-meshheading:11554455-NF-kappa B,
pubmed-meshheading:11554455-Oncogene Protein p21(ras),
pubmed-meshheading:11554455-Oxidation-Reduction,
pubmed-meshheading:11554455-Phosphorylation,
pubmed-meshheading:11554455-Protein Processing, Post-Translational,
pubmed-meshheading:11554455-Rats,
pubmed-meshheading:11554455-Recombinant Fusion Proteins,
pubmed-meshheading:11554455-Superoxide Dismutase,
pubmed-meshheading:11554455-Superoxides,
pubmed-meshheading:11554455-Transcription, Genetic,
pubmed-meshheading:11554455-Transcription Factor AP-1,
pubmed-meshheading:11554455-Transcription Factor AP-2,
pubmed-meshheading:11554455-Transcription Factors,
pubmed-meshheading:11554455-Transfection
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pubmed:year |
2001
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pubmed:articleTitle |
V-Ha-Ras overexpression induces superoxide production and alters levels of primary antioxidant enzymes.
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pubmed:affiliation |
Department of Radiology and Holden Comprehensive Cancer Center, The University of Iowa, Iowa City 52242-1181, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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