Source:http://linkedlifedata.com/resource/pubmed/id/11553667
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
2001-9-12
|
| pubmed:abstractText |
The effect of gamma-hydroxybutyric acid on extracellular glutamate levels in the hippocampus was studied by microdialysis in freely moving rats and in isolated hippocampal synaptosomes. Intra-hippocampal (CA1) perfusion with gamma-hydroxybutyric acid (10 nM-1 mM) concentration-dependently influenced glutamate levels: gamma-hydroxybutyric acid (100 and 500 nM) increased glutamate levels; 100 and 300 microM concentrations were ineffective; whereas the highest 1 mM concentration reduced local glutamate levels. The stimulant effect of gamma-hydroxybutyric acid (100 nM) was suppressed by the locally co-perfused gamma-hydroxybutyric acid receptor antagonist NCS-382 (10 microM) but not by the GABA(B) receptor antagonist CGP-35348 (500 microM). Furthermore, the gamma-hydroxybutyric acid (1 mM)-induced reduction in CA1 glutamate levels was counteracted by NCS-382 (10 microM), and it was also reversed into an increase by CGP-35348. Given alone, neither NCS-382 nor CGP-35348 modified glutamate levels. In hippocampal synaptosomes, gamma-hydroxybutyric acid (50 and 100 nM) enhanced both the spontaneous and K(+)-evoked glutamate efflux, respectively, both effects being counteracted by NCS-382 (100 nM), but not by CGP-35348 (100 microM). These findings indicate that gamma-hydroxybutyric acid exerts a concentration-dependent regulation of hippocampal glutamate transmission via two opposing mechanisms, whereby a direct gamma-hydroxybutyric acid receptor mediated facilitation is observed at nanomolar gamma-hydroxybutyric acid concentrations, and an indirect GABA(B) receptor mediated inhibition predominates at millimolar concentrations.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Anesthetics, Intravenous,
http://linkedlifedata.com/resource/pubmed/chemical/Anticonvulsants,
http://linkedlifedata.com/resource/pubmed/chemical/Benzocycloheptenes,
http://linkedlifedata.com/resource/pubmed/chemical/CGP 35348,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/GABA Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Glutamic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/NCS 382,
http://linkedlifedata.com/resource/pubmed/chemical/Organophosphorus Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium Oxybate,
http://linkedlifedata.com/resource/pubmed/chemical/Tetrodotoxin
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0022-3042
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
78
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
929-39
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:11553667-Anesthetics, Intravenous,
pubmed-meshheading:11553667-Animals,
pubmed-meshheading:11553667-Anticonvulsants,
pubmed-meshheading:11553667-Benzocycloheptenes,
pubmed-meshheading:11553667-Calcium,
pubmed-meshheading:11553667-Consciousness,
pubmed-meshheading:11553667-Extracellular Space,
pubmed-meshheading:11553667-GABA Antagonists,
pubmed-meshheading:11553667-Glutamic Acid,
pubmed-meshheading:11553667-Hippocampus,
pubmed-meshheading:11553667-Male,
pubmed-meshheading:11553667-Microdialysis,
pubmed-meshheading:11553667-Organophosphorus Compounds,
pubmed-meshheading:11553667-Potassium,
pubmed-meshheading:11553667-Rats,
pubmed-meshheading:11553667-Rats, Sprague-Dawley,
pubmed-meshheading:11553667-Sodium Oxybate,
pubmed-meshheading:11553667-Synaptic Transmission,
pubmed-meshheading:11553667-Synaptosomes,
pubmed-meshheading:11553667-Tetrodotoxin
|
| pubmed:year |
2001
|
| pubmed:articleTitle |
gamma-Hydroxybutyrate modulation of glutamate levels in the hippocampus: an in vivo and in vitro study.
|
| pubmed:affiliation |
Department of Clinical and Experimental Medicine, Pharmacology Section, University of Ferrara, Ferrara, Italy.
|
| pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
|