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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
47
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pubmed:dateCreated |
2001-11-19
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pubmed:databankReference |
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pubmed:abstractText |
Guanine nucleotide exchange factors of the Dbl family relay signals from membrane receptors to Rho family GTPases. We now demonstrate that a longer transcript of the Lbc gene encodes a chimeric molecule, which we have called AKAP-Lbc, that functions as an A-kinase-anchoring protein (AKAP) and a Rho-selective guanine nucleotide exchange factor. Expression of AKAP-Lbc in fibroblasts favors the formation of stress fibers in a Rho-dependent manner. Application of lysophosphatidic acid or selective expression of Galpha(12) enhances cellular AKAP-Lbc activation. Furthermore, biochemical studies indicate that AKAP-Lbc functions as an adaptor protein to selectively couple Galpha(12) to Rho. Thus, AKAP-Lbc anchors PKA and nucleates the assembly of a Rho-mediated signaling pathway.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
23
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pubmed:volume |
276
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
44247-57
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:11546812-3T3 Cells,
pubmed-meshheading:11546812-Animals,
pubmed-meshheading:11546812-Cell Line,
pubmed-meshheading:11546812-Cloning, Molecular,
pubmed-meshheading:11546812-Cyclic AMP-Dependent Protein Kinases,
pubmed-meshheading:11546812-DNA, Complementary,
pubmed-meshheading:11546812-GTP-Binding Proteins,
pubmed-meshheading:11546812-Humans,
pubmed-meshheading:11546812-Mice,
pubmed-meshheading:11546812-Molecular Sequence Data,
pubmed-meshheading:11546812-Mutagenesis,
pubmed-meshheading:11546812-Protein Binding,
pubmed-meshheading:11546812-Recombinant Fusion Proteins
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pubmed:year |
2001
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pubmed:articleTitle |
AKAP-Lbc anchors protein kinase A and nucleates Galpha 12-selective Rho-mediated stress fiber formation.
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pubmed:affiliation |
Howard Hughes Medical Institute, Vollum Institute, Oregon Health Sciences University, Portland, Oregon 97201, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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