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rdf:type |
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lifeskim:mentions |
umls-concept:C0015576,
umls-concept:C0031727,
umls-concept:C0086597,
umls-concept:C0178666,
umls-concept:C0271510,
umls-concept:C0330390,
umls-concept:C0919479,
umls-concept:C1298907,
umls-concept:C1546945,
umls-concept:C1546947,
umls-concept:C1546969,
umls-concept:C1548171,
umls-concept:C1549060,
umls-concept:C1549065,
umls-concept:C1549443,
umls-concept:C1549445,
umls-concept:C1705108
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pubmed:issue |
47
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pubmed:dateCreated |
2001-11-19
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pubmed:abstractText |
The insulin and the endothelin type A (ETA) receptor both can couple into the heterotrimeric G protein alpha(q/11) (Galpha(q/11)), leading to Galpha(q/11) tyrosine phosphorylation, phosphatidylinositol 3-kinase activation, and subsequent stimulation of glucose transport. In this study, we assessed the potential role of Src kinase in ET-1 signaling to glucose transport in 3T3-L1 adipocytes. Src kinase inhibitor PP2 blocked ET-1-induced Src kinase activity, Galpha(q/11) tyrosine phosphorylation, and glucose transport stimulation. To determine which Src family kinase member was involved, we microinjected anti-c-Src, -c-Fyn, or -c-Yes antibody into these cells and found that only anti-c-Yes antibody blocked GLUT4 translocation (70% decreased). Overexpression or microinjection of a dominant negative mutant (K298M) of Src kinase also inhibited ET-1-induced Galpha(q/11) tyrosine phosphorylation and GLUT4 translocation. In co-immunoprecipitation experiments, we found that beta-arrestin 1 associated with the ETA receptor in an agonist-dependent manner and that beta-arrestin 1 recruited Src kinase to a molecular complex that included the ETA receptor. Microinjection of beta-arrestin 1 antibody inhibited ET-1- but not insulin-stimulated GLUT4 translocation. In conclusion, 1) the Src kinase Yes can induce tyrosine phosphorylation of Galpha(q/11) in response to ET-1 stimulation, and 2) beta-arrestin 1 and Src kinase form a molecular complex with the ETA receptor to mediate ET-1 signaling to Galpha(q/11) with subsequent glucose transport stimulation.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Arrestins,
http://linkedlifedata.com/resource/pubmed/chemical/Endothelin-1,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Protein alpha...,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose Transporter Type 4,
http://linkedlifedata.com/resource/pubmed/chemical/Heterotrimeric GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Monosaccharide Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Muscle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-yes,
http://linkedlifedata.com/resource/pubmed/chemical/Slc2a4 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Yes1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/beta-arrestin,
http://linkedlifedata.com/resource/pubmed/chemical/src-Family Kinases
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
23
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pubmed:volume |
276
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
43663-7
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:11546805-3T3 Cells,
pubmed-meshheading:11546805-Animals,
pubmed-meshheading:11546805-Arrestins,
pubmed-meshheading:11546805-Biological Transport,
pubmed-meshheading:11546805-Endothelin-1,
pubmed-meshheading:11546805-GTP-Binding Protein alpha Subunits, Gq-G11,
pubmed-meshheading:11546805-Glucose,
pubmed-meshheading:11546805-Glucose Transporter Type 4,
pubmed-meshheading:11546805-Heterotrimeric GTP-Binding Proteins,
pubmed-meshheading:11546805-Mice,
pubmed-meshheading:11546805-Microscopy, Fluorescence,
pubmed-meshheading:11546805-Monosaccharide Transport Proteins,
pubmed-meshheading:11546805-Muscle Proteins,
pubmed-meshheading:11546805-Proto-Oncogene Proteins,
pubmed-meshheading:11546805-Proto-Oncogene Proteins c-yes,
pubmed-meshheading:11546805-Signal Transduction,
pubmed-meshheading:11546805-src-Family Kinases
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pubmed:year |
2001
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pubmed:articleTitle |
beta -Arrestin-mediated recruitment of the Src family kinase Yes mediates endothelin-1-stimulated glucose transport.
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pubmed:affiliation |
Department of Medicine, Division of Endocrinology and Metabolism, University of California, San Diego, La Jolla, California 92093-0673, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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