Linked Life Data

11544345

Source:http://linkedlifedata.com/resource/pubmed/id/11544345

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2001-9-6
pubmed:abstractText
Numerous clinical and epidemiological studies link enteroviruses such as the Coxsackie virus group with the autoimmune disease type 1 diabetes mellitus (DM). In addition, there are reports that patients with type 1 DM are characterized by skewing of TCR Vbeta chain selection among peripheral blood and intraislet T lymphocytes. To examine these issues, we analyzed TCR Vbeta chain-specific up-regulation of the early T cell activation marker, CD69, on CD4 T cells after incubation with Coxsackievirus B4 (CVB4) Ags. CD4 T cells bearing the Vbeta chains 2, 7, and 8 were the most frequently activated by CVB4. Up-regulation of CD69 by different TCR families was significantly more frequent in new onset type 1 DM patients (p = 0.04), 100% of whom (n = 8) showed activation of CD4 T cells bearing Vbeta8, compared with 50% of control subjects (n = 8; p = 0.04). T cell proliferation after incubation with CVB4 Ags required live, nonfixed APCs, suggesting that the selective expansion of CD4 T cells with particular Vbeta chains resulted from conventional antigen processing and presentation rather than superantigen activity. Heteroduplex analysis of TCR Vbeta chain usage after CVB4 stimulation indicated a relatively polyclonal, rather than oligo- or monoclonal response to viral Ags. These results provide evidence that new-onset patients with type 1 DM and healthy controls are primed against CVB4, and that CD4 T cell responses to the virus have a selective TCR Vbeta chain usage which is driven by viral Ags rather than a superantigen.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
167
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3513-20
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:11544345-Adult, pubmed-meshheading:11544345-Antigen Presentation, pubmed-meshheading:11544345-Antigen-Presenting Cells, pubmed-meshheading:11544345-Antigens, CD, pubmed-meshheading:11544345-Antigens, Differentiation, T-Lymphocyte, pubmed-meshheading:11544345-Antigens, Viral, pubmed-meshheading:11544345-Autoimmune Diseases, pubmed-meshheading:11544345-CD4-Positive T-Lymphocytes, pubmed-meshheading:11544345-Cell Division, pubmed-meshheading:11544345-Coculture Techniques, pubmed-meshheading:11544345-Diabetes Mellitus, Type 1, pubmed-meshheading:11544345-Enterovirus B, Human, pubmed-meshheading:11544345-Enterovirus Infections, pubmed-meshheading:11544345-Female, pubmed-meshheading:11544345-Gene Rearrangement, beta-Chain T-Cell Antigen Receptor, pubmed-meshheading:11544345-Heteroduplex Analysis, pubmed-meshheading:11544345-Humans, pubmed-meshheading:11544345-Lectins, C-Type, pubmed-meshheading:11544345-Lymphocyte Activation, pubmed-meshheading:11544345-Male, pubmed-meshheading:11544345-Receptors, Antigen, T-Cell, alpha-beta, pubmed-meshheading:11544345-Superantigens, pubmed-meshheading:11544345-T-Lymphocyte Subsets, pubmed-meshheading:11544345-Up-Regulation
pubmed:year
2001
pubmed:articleTitle
T cell activation by coxsackievirus B4 antigens in type 1 diabetes mellitus: evidence for selective TCR Vbeta usage without superantigenic activity.
pubmed:affiliation
Department of Immunology, Guy's, King's and St. Thomas' School of Medicine, London, United Kingdom.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't