Linked Life Data

11540813

Source:http://linkedlifedata.com/resource/pubmed/id/11540813

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1998-8-28
pubmed:abstractText
Many gram-positive bacteria form substituted polymers of glycerol and ribitol phosphate esters known as teichoic acids. Utilizing the relative specificity of cold concentrated hydrofluoric acid in the hydrolysis of polyphosphate esters it proved possible to quantitatively assay the teichoic acid-derived glycerol and ribitol from gram-positive bacteria added to various soils and sediments. The lipids are first removed from the soils or sediments with a one phase chloroform-methanol extraction and the lipid extracted residue is hydrolyzed with cold concentrated hydrofluoric acid. To achieve maximum recovery of the teichoic acid ribitol, a second acid hydrolysis of the aqueous extract is required. The glycerol and ribitol are then acetylated after neutralization and analyzed by capillary gas-liquid chromatography. This technique together with measures of the total phospholipid, the phospholipid fatty acid, the muramic acid and the hydroxy fatty acids of the lipopolysaccharide lipid A of the gram-negative bacteria makes it possible to describe the community structure environmental samples. The proportion of gram-positive bacteria measured as the teichoic acid glycerol and ribitol is higher in soils than in sediments and increases with depth in both.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
S
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0167-7012
pubmed:author
pubmed:issnType
Print
pubmed:volume
2
pubmed:owner
NASA
pubmed:authorsComplete
Y
pubmed:pagination
165-76
pubmed:dateRevised
2007-8-1
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Determination of the gram-positive bacterial content of soils and sediments by analysis of teichoic acid components.
pubmed:affiliation
Department of Biological Science, Florida State University, Tallahassee 32306, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.