Source:http://linkedlifedata.com/resource/pubmed/id/11539117
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:dateCreated |
1996-10-7
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| pubmed:abstractText |
The prototype of a miniaturized cell cultivation instrument for animal cell culture experiments aboard Spacelab is presented (Dynamic cell culture system: DCCS). The cell chamber is completely filled and has a working volume of 200 microliters. Medium exchange is achieved with a self-powered osmotic pump (flowrate 1 microliter h-1). The reservoir volume of culture medium is 230 microliters. The system is neither mechanically stirred nor equipped with sensors. Hamster kidney (Hak) cells growing on Cytodex 3 microcarriers were used to test the biological performance of the DCCS. Growth characteristics in the DCCS, as judged by maximal cell density, glucose consumption, lactic acid secretion and pH, were similar to those in cell culture tubes.
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| pubmed:keyword | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
S
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:issn |
0168-1656
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
7
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| pubmed:owner |
NASA
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
217-28
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| pubmed:dateRevised |
2007-11-15
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| pubmed:meshHeading |
pubmed-meshheading:11539117-Animals,
pubmed-meshheading:11539117-Biotechnology,
pubmed-meshheading:11539117-Cell Count,
pubmed-meshheading:11539117-Cell Culture Techniques,
pubmed-meshheading:11539117-Cell Division,
pubmed-meshheading:11539117-Cricetinae,
pubmed-meshheading:11539117-Culture Media,
pubmed-meshheading:11539117-Cytological Techniques,
pubmed-meshheading:11539117-Diffusion Chambers, Culture,
pubmed-meshheading:11539117-Equipment Design,
pubmed-meshheading:11539117-Evaluation Studies as Topic,
pubmed-meshheading:11539117-Glucose,
pubmed-meshheading:11539117-Hydrogen-Ion Concentration,
pubmed-meshheading:11539117-Kidney,
pubmed-meshheading:11539117-Lactates,
pubmed-meshheading:11539117-Lactic Acid,
pubmed-meshheading:11539117-Space Flight,
pubmed-meshheading:11539117-Weightlessness
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| pubmed:year |
1988
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| pubmed:articleTitle |
Dynamic cell culture system: a new cell cultivation instrument for biological experiments in space.
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| pubmed:affiliation |
Laboratorium für Biochemie IV, ETH-Zürich, Switzerland.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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