Linked Life Data

11537409

Source:http://linkedlifedata.com/resource/pubmed/id/11537409

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1998-8-28
pubmed:abstractText
2'-d-5'-GMP and 2'-d-5'-AMP bind 2 times more strongly to montmorillonite 22A than do 2'-d-5'-CMP and 5'-TMP. The dinucleotide d(pG)2 forms in 9.2% yield and the cyclic dinucleotide c(dpG)2 in 5.4% yield in the reaction of 2'-d-5'-GMP with EDAC in the presence of montmorillonite 22A. The yield of d(pC)2 (2.0%) is significantly lower but comparable to that obtained from 5'-TMP. The yield of dimers which contain the phosphodiester bond decreases as the reaction medium is changed from 0.2 M NaCl to a mixture of 0.2 M NaCl and 0.075 M MgCl2. A low yield of d(pA)2 was observed in the condensation reaction of 5'-ImdpA on montmorillonite 22A. The cyclic nucleotide (3',5'-cdAMP) was obtained in 14% yield from 3'-ImdpA. The yield of d(pA)2 obtained when EDAC is used as the condensing agent increases with increasing iron content of the Na(+)-montmorillonite used as a catalyst. Evidence is presented which shows that the acidity of the Na(+)-montmorillonite is a necessary but not sufficient factor for the montmorillonite catalysis of phosphodiester bond formation.
pubmed:keyword
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
S
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/1-ethyl-3-(3-dimethylaminoethyl)carb..., http://linkedlifedata.com/resource/pubmed/chemical/2'-deoxy-5'-adenosine monophosphate, http://linkedlifedata.com/resource/pubmed/chemical/5'-Guanylic Acid, http://linkedlifedata.com/resource/pubmed/chemical/Bentonite, http://linkedlifedata.com/resource/pubmed/chemical/Carbodiimides, http://linkedlifedata.com/resource/pubmed/chemical/Cytidine Monophosphate, http://linkedlifedata.com/resource/pubmed/chemical/Deoxyadenine Nucleotides, http://linkedlifedata.com/resource/pubmed/chemical/Deoxyribonucleotides, http://linkedlifedata.com/resource/pubmed/chemical/Imidazoles, http://linkedlifedata.com/resource/pubmed/chemical/Iron, http://linkedlifedata.com/resource/pubmed/chemical/Magnesium Chloride, http://linkedlifedata.com/resource/pubmed/chemical/Phosphoric Acid Esters, http://linkedlifedata.com/resource/pubmed/chemical/Sodium Chloride
pubmed:status
MEDLINE
pubmed:issn
0169-6149
pubmed:author
pubmed:issnType
Print
pubmed:volume
20
pubmed:owner
NASA
pubmed:authorsComplete
Y
pubmed:pagination
279-91
pubmed:dateRevised
2007-8-1
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Oligomerization reactions of deoxyribonucleotides on montmorillonite clay: the effect of mononucleotide structure, phosphate activation and montmorillonite composition on phosphodiester bond formation.
pubmed:affiliation
Rensselaer Polytechnic Institute, Troy, NY 12180-3590, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.