Linked Life Data

11536873

Source:http://linkedlifedata.com/resource/pubmed/id/11536873

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1999-6-20
pubmed:abstractText
The N-1-naphthylphthalamic acid (NPA)-binding protein is part of the auxin efflux carrier, the protein complex that controls polar auxin transport in plant tissues. This study tested the hypothesis that the NPA-binding protein (NBP) is associated with the actin cytoskeleton in vitro and that an intact actin cytoskeleton is required for polar auxin transport in vivo. Cytoskeletal polymerization was altered in extracts of zucchini hypocotyls with reagents that stabilized either the polymeric or monomeric forms of actin or tubulin. Phalloidin treatment altered actin polymerization, as demonstrated by immunoblot analyses following native and denaturing electrophoresis. Phalloidin increased both filamentous actin (F-actin) and NPA-binding activity, while cytochalasin D and Tris decreased both F-actin and NPA-binding activity in cytoskeletal pellets. The microtubule stabilizing drug taxol increased pelletable tubulin, but did not alter either the amount of pelletable actin or NPA-binding activity. Treatment of etiolated zucchini hypocotyls with cytochalasin D decreased the amount of auxin transport and its regulation by NPA. These experimental results are consistent with an in vitro actin cytoskeletal association of the NPA-binding protein and with the requirement of an intact actin cytoskeleton for maximal polar auxin transport in vivo.
pubmed:keyword
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
S
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Actins, http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Cytochalasin D, http://linkedlifedata.com/resource/pubmed/chemical/Herbicides, http://linkedlifedata.com/resource/pubmed/chemical/Indoleacetic Acids, http://linkedlifedata.com/resource/pubmed/chemical/Phalloidine, http://linkedlifedata.com/resource/pubmed/chemical/Phthalimides, http://linkedlifedata.com/resource/pubmed/chemical/Plant Growth Regulators, http://linkedlifedata.com/resource/pubmed/chemical/Plant Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Tromethamine, http://linkedlifedata.com/resource/pubmed/chemical/alpha-naphthylphthalamic acid, http://linkedlifedata.com/resource/pubmed/chemical/indoleacetic acid
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0960-7412
pubmed:author
pubmed:issnType
Print
pubmed:volume
13
pubmed:owner
NASA
pubmed:authorsComplete
Y
pubmed:pagination
291-301
pubmed:dateRevised
2007-8-1
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
In vitro and in vivo evidence for actin association of the naphthylphthalamic acid-binding protein from zucchini hypocotyls.
pubmed:affiliation
Wake Forest University, Department of Biology, Winston-Salem, NC 27109, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.