Linked Life Data

11532124

Source:http://linkedlifedata.com/resource/pubmed/id/11532124

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2001-9-4
pubmed:abstractText
Virulence gene expression in enteropathogenic Escherichia coli (EPEC) is governed by a combination of environmental factors and virulence regulators. These factors control the expression of the bundle-forming pili (BFP), intimin, the type III secretion apparatus and the secreted proteins EspA, EspB, EspD and Tir. Expression of the bfp genes occurs for a short period in early exponential phase during growth in tissue culture medium. The nucleoid-associated regulator protein, Fis, is also expressed transiently during this period. To determine whether Fis was responsible for the growth phase-dependent expression of bfp, fis was deleted from the EPEC strain E2348/69S. Paradoxically, the Delta fis mutant retained the ability to colonize HEp-2 cells in a characteristic localized adherence pattern, and Fis was found negatively to regulate the expression of BFP. However, the Delta fis mutant failed to induce the accretion of filamentous actin, which is associated with attaching and effacing lesions. Using a combination of Western blotting and a novel multiplex primer extension assay (MPEA), we showed that, although the expression of intimin and Tir was not affected, transcription of the LEE4 operon encoding espADB and the virulence activator, Ler, were found to be Fis dependent.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0950-382X
pubmed:author
pubmed:issnType
Print
pubmed:volume
41
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
549-59
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11532124-Adhesins, Bacterial, pubmed-meshheading:11532124-Blotting, Western, pubmed-meshheading:11532124-Carrier Proteins, pubmed-meshheading:11532124-Escherichia coli, pubmed-meshheading:11532124-Escherichia coli Proteins, pubmed-meshheading:11532124-Factor For Inversion Stimulation Protein, pubmed-meshheading:11532124-Fimbriae, Bacterial, pubmed-meshheading:11532124-Fluorescent Antibody Technique, Indirect, pubmed-meshheading:11532124-Gene Expression Regulation, Bacterial, pubmed-meshheading:11532124-Humans, pubmed-meshheading:11532124-Integration Host Factors, pubmed-meshheading:11532124-Mutation, pubmed-meshheading:11532124-Operon, pubmed-meshheading:11532124-Phenotype, pubmed-meshheading:11532124-RNA, Bacterial, pubmed-meshheading:11532124-Tumor Cells, Cultured, pubmed-meshheading:11532124-Virulence
pubmed:year
2001
pubmed:articleTitle
Role of the nucleoid-associated protein Fis in the regulation of virulence properties of enteropathogenic Escherichia coli.
pubmed:affiliation
Department of Microbiology and Immunology, School of Medicine, Maurice Shock Building, PO Box 138, University Road, Leicester LE1 9HN, UK. martin.goldberg@bbsrc.ac.uk
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't