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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2001-8-31
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pubmed:abstractText |
The glucocorticoid receptor (GR) is a ligand dependent transcription factor, which regulates the transcription of multiple hormone-dependent genes. The transcriptional regulation by GR takes place by interaction of GR with the basal transcription machinery and by recruiting glucocorticoid receptor interacting proteins (GRIPs). Previously we identified hnRNP U/SAF-A as a factor interfering with GR-dependent transcription by repressing glucocorticoid induced activation. To gain insight into the mechanisms that govern this interference, we have now investigated the transcription of GR-dependent reporter genes in Ltk(-) cells transiently transfected with a variety of hnRNP U constructs. We demonstrate that a hnRNP U construct lacking the GR-binding domain acts as a dominant negative factor that now enhances GR-driven transcription. In addition, hnRNP U repression of glucocorticoid induced transcription was found to be dependent on the amount of cotransfected GR, where a high amount of GR leads to ligand-inducible repression of GR-dependent reporter gene activity by hnRNP U, whereas low amounts of GR showed nearly no effect. The relative concentrations of GR, hnRNP U and DNA-binding sites for GR are important for the effect of hnRNP U on transcription, suggesting a model where hnRNP-U acts as a storage site for intranuclear GR.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0960-0760
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
78
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
59-65
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pubmed:dateRevised |
2006-5-1
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pubmed:meshHeading |
pubmed-meshheading:11530285-Binding Sites,
pubmed-meshheading:11530285-Cell Line,
pubmed-meshheading:11530285-Cell Nucleus,
pubmed-meshheading:11530285-Cell Separation,
pubmed-meshheading:11530285-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:11530285-Eukaryotic Cells,
pubmed-meshheading:11530285-Flow Cytometry,
pubmed-meshheading:11530285-Genes, Dominant,
pubmed-meshheading:11530285-Genes, Reporter,
pubmed-meshheading:11530285-Glucocorticoids,
pubmed-meshheading:11530285-Heterogeneous-Nuclear Ribonucleoprotein U,
pubmed-meshheading:11530285-Heterogeneous-Nuclear Ribonucleoproteins,
pubmed-meshheading:11530285-Humans,
pubmed-meshheading:11530285-Ligands,
pubmed-meshheading:11530285-Models, Genetic,
pubmed-meshheading:11530285-Mutation,
pubmed-meshheading:11530285-Plasmids,
pubmed-meshheading:11530285-Protein Binding,
pubmed-meshheading:11530285-Protein Structure, Tertiary,
pubmed-meshheading:11530285-RNA Polymerase II,
pubmed-meshheading:11530285-Ribonucleoproteins,
pubmed-meshheading:11530285-Transcription, Genetic,
pubmed-meshheading:11530285-Transfection
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pubmed:year |
2001
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pubmed:articleTitle |
Effects of the heterogeneous nuclear ribonucleoprotein U (hnRNP U/SAF-A) on glucocorticoid-dependent transcription in vivo.
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pubmed:affiliation |
Institut für Genetik der Justus-Liebig-Universität, Heinrich-Buff-Ring 58-62, D-35392 Giessen, Germany.
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pubmed:publicationType |
Journal Article
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