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rdf:type |
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lifeskim:mentions |
umls-concept:C0003483,
umls-concept:C0017262,
umls-concept:C0034693,
umls-concept:C0034721,
umls-concept:C0038435,
umls-concept:C0058402,
umls-concept:C0166825,
umls-concept:C0205263,
umls-concept:C0311417,
umls-concept:C1135918,
umls-concept:C1150587,
umls-concept:C1335273,
umls-concept:C1367731,
umls-concept:C1704816,
umls-concept:C1705632,
umls-concept:C1707310,
umls-concept:C1710082
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pubmed:issue |
3
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pubmed:dateCreated |
2001-8-31
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pubmed:abstractText |
The present study was undertaken in an attempt to clarify the pathway by which hyperosmotic stress induces HB-EGF gene expression in rat aortic smooth muscle cells (RASMC). Hyperosmotic stress induced by a high concentration of glucose or mannitol resulted in an increase in HB-EGF mRNA level in a dose- and time-dependent manner. HB-EGF induction was blocked by curcumin, a c-jun/fos antisense oligonucleotide and a dominant-negative mutant of JNK1. Electrophoretic mobility shift assay also showed the involvement of AP-1 in HB-EGF gene expression by glucose. In addition, hyperosmotic stress induced rapid phosphorylation of Pyk2 in RASMC. TPA and calcium chelating agents (BAPTA-AM and EGTA) blocked Pyk2 phosphorylation and HB-EGF gene expression. Furthermore, HB-EGF gene expression and JNK activation by hyperosmotic stress were sensitive to PP2, an Src kinase-specific inhibitor. These findings indicate that hyperosmotic stress activates JNK via calcium-Pyk2 signaling cascades, which in turn induce HB-EGF gene expression.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Epidermal Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Focal Adhesion Kinase 2,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/JNK Mitogen-Activated Protein...,
http://linkedlifedata.com/resource/pubmed/chemical/Mannitol,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Ptk2b protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor AP-1,
http://linkedlifedata.com/resource/pubmed/chemical/heparin-binding EGF-like growth...,
http://linkedlifedata.com/resource/pubmed/chemical/src-Family Kinases
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0021-924X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
130
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
351-8
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:11530010-Animals,
pubmed-meshheading:11530010-Aorta,
pubmed-meshheading:11530010-Calcium,
pubmed-meshheading:11530010-Epidermal Growth Factor,
pubmed-meshheading:11530010-Focal Adhesion Kinase 2,
pubmed-meshheading:11530010-Gene Expression,
pubmed-meshheading:11530010-Glucose,
pubmed-meshheading:11530010-Humans,
pubmed-meshheading:11530010-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:11530010-JNK Mitogen-Activated Protein Kinases,
pubmed-meshheading:11530010-Mannitol,
pubmed-meshheading:11530010-Mitogen-Activated Protein Kinases,
pubmed-meshheading:11530010-Muscle, Smooth, Vascular,
pubmed-meshheading:11530010-Osmotic Pressure,
pubmed-meshheading:11530010-Protein-Tyrosine Kinases,
pubmed-meshheading:11530010-RNA, Messenger,
pubmed-meshheading:11530010-Rats,
pubmed-meshheading:11530010-Rats, Wistar,
pubmed-meshheading:11530010-Signal Transduction,
pubmed-meshheading:11530010-Transcription Factor AP-1,
pubmed-meshheading:11530010-src-Family Kinases
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pubmed:year |
2001
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pubmed:articleTitle |
Osmotic stress induces HB-EGF gene expression via Ca(2+)/Pyk2/JNK signal cascades in rat aortic smooth muscle cells.
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pubmed:affiliation |
Department of Biochemistry, Osaka University Medical School, Yamadaoka, Suita, Osaka 565-0871, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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