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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2001-8-30
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pubmed:abstractText |
Disruption of the APC/beta-catenin/Tcf pathway has been proposed as an important step in the development of cancer. The Tcf-4 transcription factor gene was reported to be one of the targets of microsatellite instability (MSI) in colorectal cancers in with MSI. We carried out a sequencing analysis of the Tcf-4 gene in 41 Japanese patients with gastrointestinal tumors with MSI as well as in cancer cell lines. Three of 21 (14.3%) colorectal tumors with MSI contained a mutant Tcf-4 gene encoding 1-bp deletion in an (A)9 repeat, leading to carboxyl terminal truncation of Tcf-4 protein. No Tcf-4 mutations were detected in 20 gastric tumors with MSI, or in gastric cancer cell lines. Additionally, we found a novel transcript of the Tcf-4 gene which contained a segment of 73 bp in front of the (A)9 repeat of the Tcf-4 coding sequence. Sequencing analysis revealed that the inserted fragment was 60% homologous to that of exon IXA of the Tcf-1 gene. It is of interest that this insertion resulted in truncation of Tcf-4, similar to the 1-bp deletion in the (A)9 repeat. Therefore, in part of the Japanese colorectal tumors with MSI, frameshift mutations in Tcf-4 may be of functional significance. Functional alterations in the Tcf-4 signaling network in gastrointestinal tumorigenesis require further investigations.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
0030-2414
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2001 S. Karger AG, Basel
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pubmed:issnType |
Print
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pubmed:volume |
61
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
156-61
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:11528255-Adenocarcinoma,
pubmed-meshheading:11528255-Alternative Splicing,
pubmed-meshheading:11528255-Amino Acid Sequence,
pubmed-meshheading:11528255-Base Sequence,
pubmed-meshheading:11528255-Colorectal Neoplasms,
pubmed-meshheading:11528255-DNA, Neoplasm,
pubmed-meshheading:11528255-DNA Mutational Analysis,
pubmed-meshheading:11528255-Gastrointestinal Neoplasms,
pubmed-meshheading:11528255-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:11528255-Humans,
pubmed-meshheading:11528255-Japan,
pubmed-meshheading:11528255-Microsatellite Repeats,
pubmed-meshheading:11528255-Molecular Sequence Data,
pubmed-meshheading:11528255-Neoplasm Proteins,
pubmed-meshheading:11528255-Protein Isoforms,
pubmed-meshheading:11528255-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:11528255-Sequence Alignment,
pubmed-meshheading:11528255-Sequence Analysis, DNA,
pubmed-meshheading:11528255-Sequence Deletion,
pubmed-meshheading:11528255-Sequence Homology, Amino Acid,
pubmed-meshheading:11528255-Stomach Neoplasms,
pubmed-meshheading:11528255-TCF Transcription Factors,
pubmed-meshheading:11528255-Transcription Factor 7-Like 2 Protein,
pubmed-meshheading:11528255-Transcription Factors,
pubmed-meshheading:11528255-Transcriptional Activation,
pubmed-meshheading:11528255-Tumor Cells, Cultured
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pubmed:year |
2001
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pubmed:articleTitle |
Genetic alterations in the human Tcf-4 gene in Japanese patients with sporadic gastrointestinal cancers with microsatellite instability.
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pubmed:affiliation |
Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan. hsaeki@med.kyushu-u.ac.jp
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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