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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
36
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pubmed:dateCreated |
2001-8-29
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pubmed:abstractText |
Multiple endocrine neoplasia type 1 is an autosomal dominant tumor syndrome. Manifestations include neoplasms of the parathyroid glands, enteropancreatic neuroendocrine cells, and the anterior pituitary gland. The MEN1 tumor suppressor gene encodes menin, a 610 amino acid nuclear protein without sequence homology to other proteins. To elucidate menin function, we used immunoprecipitation to identify interacting proteins. The NF-kappaB proteins p50, p52 and p65 were found to interact specifically and directly with menin in vitro and in vivo. The region of NF-kappaB proteins sufficient for binding to menin is the N-terminus. Furthermore, amino acids 305-381 of menin are essential for this binding. Menin represses p65-mediated transcriptional activation on NF-kappaB sites in a dose-dependent and specific manner. Also, PMA (phorbol 12-myristate 13-acetate)-stimulated NF-kappaB activation is suppressed by menin. These observations suggest that menin's ability to interact with NF-kappaB proteins and its modulation of NF-kappaB transactivation contribute to menin's tumor suppressor function.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0950-9232
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pubmed:author |
pubmed-author:AgarwalS KSK,
pubmed-author:BilimoriaK YKY,
pubmed-author:BurnsA LAL,
pubmed-author:ChandrasekharappaS CSC,
pubmed-author:CollinsF SFS,
pubmed-author:GuruS CSC,
pubmed-author:HeppnerCC,
pubmed-author:KesterMM,
pubmed-author:MarxS JSJ,
pubmed-author:SpiegelA MAM,
pubmed-author:WhittyL JLJ
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pubmed:issnType |
Print
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pubmed:day |
16
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|
pubmed:volume |
20
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4917-25
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:11526476-Animals,
pubmed-meshheading:11526476-COS Cells,
pubmed-meshheading:11526476-Cell Line,
pubmed-meshheading:11526476-Genes, Tumor Suppressor,
pubmed-meshheading:11526476-Glutathione Transferase,
pubmed-meshheading:11526476-HeLa Cells,
pubmed-meshheading:11526476-Humans,
pubmed-meshheading:11526476-NF-kappa B,
pubmed-meshheading:11526476-Neoplasm Proteins,
pubmed-meshheading:11526476-Precipitin Tests,
pubmed-meshheading:11526476-Protein Structure, Tertiary,
pubmed-meshheading:11526476-Proto-Oncogene Proteins,
pubmed-meshheading:11526476-Tetradecanoylphorbol Acetate,
pubmed-meshheading:11526476-Transcriptional Activation
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pubmed:year |
2001
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pubmed:articleTitle |
The tumor suppressor protein menin interacts with NF-kappaB proteins and inhibits NF-kappaB-mediated transactivation.
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pubmed:affiliation |
Metabolic Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, USA.
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pubmed:publicationType |
Journal Article
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