Linked Life Data

11526135

Source:http://linkedlifedata.com/resource/pubmed/id/11526135

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2001-8-29
pubmed:abstractText
The emergence of coagulase-negative staphylococci not only as human pathogens but also as reservoirs of antibiotic resistance determinants requires the deployment and development of methods for their rapid and reliable identification. Internal transcribed spacer-PCR (ITS-PCR) was used to identify a collection of 617 clinical staphylococcal isolates. The amplicons were resolved in high-resolution agarose gels and visually compared with the patterns obtained for the control strains of 29 staphylococcal species. Of the 617 isolates studied, 592 (95.95%) were identified by ITS-PCR and included 11 species: 302 isolates of Staphylococcus epidermidis, 157 of S. haemolyticus, 79 of S. aureus, 21 of S. hominis, 14 of S. saprophyticus, 8 of S. warneri, 6 of S. simulans, 2 of S. lugdunensis, and 1 each of S. caprae, S. carnosus, and S. cohnii. All species analyzed had unique ITS-PCR patterns, although some were very similar, namely, the group S. saprophyticus, S. cohnii, S. gallinarum, S. xylosus, S. lentus, S. equorum, and S. chromogenes, the pair S. schleiferi and S. vitulus, and the pair S. piscifermentans and S. carnosus. Four species, S. aureus, S. caprae, S. haemolyticus, and S. lugdunensis, showed polymorphisms on their ITS-PCR patterns. ITS-PCR proved to be a valuable alternative for the identification of staphylococci, offering, within the same response time and at lower cost, higher reliability than the currently available commercial systems.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11526135-10703711, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526135-1726852, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526135-7773419, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526135-8039656, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526135-8118401, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526135-8476298, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526135-8501235, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526135-8581168, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526135-9011049, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526135-9157147, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526135-9158793, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526135-9387227, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526135-9569001, http://linkedlifedata.com/resource/pubmed/commentcorrection/11526135-9734063
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
39
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3099-103
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
Identification of clinical staphylococcal isolates from humans by internal transcribed spacer PCR.
pubmed:affiliation
Molecular Genetics Laboratory, Instituto de Tecnologia Química e Biológica da Universidade Nova de Lisboa, Oeiras, Portugal.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Evaluation Studies